热休克蛋白 70 伴侣系统的作用机制。
Mechanisms of the Hsp70 chaperone system.
机构信息
Department of Biochemistry, McGill University, 3655 Promenade Sir William Osler, Montreal, QC H3G 1Y6, Canada.
出版信息
Biochem Cell Biol. 2010 Apr;88(2):291-300. doi: 10.1139/o09-175.
Abstract
Molecular chaperones of the Hsp70 family have diverse functions in cells. They assist the folding of newly synthesized and stress-denatured proteins, as well as the import of proteins into organelles, and the dissociation of aggregated proteins. The well-conserved Hsp70 chaperones are ATP dependent: binding and hydrolysis of ATP regulates their interactions with unfolded polypeptide substrates, and ATPase cycling is necessary for their function. All cellular functions of Hsp70 chaperones use the same mechanism of ATP-driven polypeptide binding and release. The Hsp40 co-chaperones stimulate ATP hydrolysis by Hsp70 and the type 1 Hsp40 proteins are conserved from Escherichia coli to humans. Various nucleotide exchange factors also promote the Hsp70 ATPase cycle. Recent advances have added to our understanding of the Hsp70 mechanism at a molecular level.
摘要
Hsp70 家族的分子伴侣在细胞中具有多种功能。它们协助新合成和应激变性的蛋白质折叠，以及蛋白质进入细胞器，以及聚集蛋白质的解离。高度保守的 Hsp70 伴侣是 ATP 依赖性的：ATP 的结合和水解调节它们与未折叠多肽底物的相互作用，并且 ATP 酶循环是其功能所必需的。Hsp70 伴侣的所有细胞功能都使用相同的机制，即 ATP 驱动的多肽结合和释放。Hsp40 共伴侣刺激 Hsp70 的 ATP 水解，并且从大肠杆菌到人类都保守了类型 1 Hsp40 蛋白。各种核苷酸交换因子也促进 Hsp70 ATP 酶循环。最近的进展增加了我们对 Hsp70 机制的分子水平的理解。
相似文献
1
Mechanisms of the Hsp70 chaperone system.热休克蛋白 70 伴侣系统的作用机制。
Biochem Cell Biol. 2010 Apr;88(2):291-300. doi: 10.1139/o09-175.
2
The dissociation of ATP from hsp70 of Saccharomyces cerevisiae is stimulated by both Ydj1p and peptide substrates.来自酿酒酵母热休克蛋白70（hsp70）的三磷酸腺苷（ATP）的解离受到Ydj1p和肽底物的双重刺激。
J Biol Chem. 1995 May 5;270(18):10412-9. doi: 10.1074/jbc.270.18.10412.
3
Regulation of ATPase and chaperone cycle of DnaK from Thermus thermophilus by the nucleotide exchange factor GrpE.嗜热栖热菌中核苷酸交换因子GrpE对DnaK的ATP酶及伴侣循环的调控
J Mol Biol. 2001 Feb 2;305(5):1173-83. doi: 10.1006/jmbi.2000.4373.
4
A disulfide-bonded DnaK dimer is maintained in an ATP-bound state.一个二硫键连接的DnaK二聚体维持在ATP结合状态。
Cell Stress Chaperones. 2017 Mar;22(2):201-212. doi: 10.1007/s12192-016-0752-y. Epub 2016 Dec 14.
5
Human Hsp70 molecular chaperone binds two calcium ions within the ATPase domain.人类热休克蛋白70（Hsp70）分子伴侣在ATP酶结构域内结合两个钙离子。
Structure. 1997 Mar 15;5(3):403-14. doi: 10.1016/s0969-2126(97)00197-4.
6
Nucleotide Exchange Factors for Hsp70 Molecular Chaperones: GrpE, Hsp110/Grp170, HspBP1/Sil1, and BAG Domain Proteins.Hsp70 分子伴侣的核苷酸交换因子：GroE、Hsp110/Grp170、HspBP1/Sil1 和 BAG 结构域蛋白。
Subcell Biochem. 2023;101:1-39. doi: 10.1007/978-3-031-14740-1_1.
7
Thermal adaptation of the yeast mitochondrial Hsp70 system is regulated by the reversible unfolding of its nucleotide exchange factor.酵母线粒体热休克蛋白70（Hsp70）系统的热适应性由其核苷酸交换因子的可逆去折叠调控。
J Mol Biol. 2006 May 19;358(5):1367-77. doi: 10.1016/j.jmb.2006.03.027. Epub 2006 Mar 29.
8
Hsp70 chaperones: cellular functions and molecular mechanism.热休克蛋白70伴侣蛋白：细胞功能与分子机制
Cell Mol Life Sci. 2005 Mar;62(6):670-84. doi: 10.1007/s00018-004-4464-6.
9
Binding of a small molecule at a protein-protein interface regulates the chaperone activity of hsp70-hsp40.小分子在蛋白质-蛋白质界面的结合调节 hsp70-hsp40 的伴侣活性。
ACS Chem Biol. 2010 Jun 18;5(6):611-22. doi: 10.1021/cb1000422.
10
The ATP hydrolysis-dependent reaction cycle of the Escherichia coli Hsp70 system DnaK, DnaJ, and GrpE.大肠杆菌Hsp70系统DnaK、DnaJ和GrpE的ATP水解依赖性反应循环。
Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10345-9. doi: 10.1073/pnas.91.22.10345.
引用本文的文献
1
Lysosomal Repair in Health and Disease.健康与疾病中的溶酶体修复
J Cell Physiol. 2025 May;240(5):e70044. doi: 10.1002/jcp.70044.
2
In silico approach on structural and functional characterization of heat shock protein from Sulfobacillus acidophilus.嗜酸硫化芽孢杆菌热休克蛋白结构与功能表征的计算机模拟方法
J Appl Genet. 2025 Apr 15. doi: 10.1007/s13353-025-00964-6.
3
Structural insights into GrpEL1-mediated nucleotide and substrate release of human mitochondrial Hsp70.人线粒体Hsp70中GrpEL1介导的核苷酸和底物释放的结构见解
Nat Commun. 2024 Dec 30;15(1):10815. doi: 10.1038/s41467-024-54499-1.
4
Quantitative proteomic analysis of residual host cell protein retention across adeno-associated virus affinity chromatography.腺相关病毒亲和色谱法中残留宿主细胞蛋白保留的定量蛋白质组学分析
Mol Ther Methods Clin Dev. 2024 Nov 18;32(4):101383. doi: 10.1016/j.omtm.2024.101383. eCollection 2024 Dec 12.
5
Impact of heat stress on physiological characteristics and expression of heat shock proteins (HSPs) in groundnut ( L.).热胁迫对花生（ ）生理特性及热休克蛋白（HSPs）表达的影响 。
注：原文括号内“( L.)”内容不完整，未明确具体信息。
Physiol Mol Biol Plants. 2024 Oct;30(10):1691-1706. doi: 10.1007/s12298-024-01520-y. Epub 2024 Oct 29.
6
Molecular characterization of heat shock protein 70 and 90 genes and their expression analysis in air-breathing magur catfish (Clarias magur) while exposed to zinc oxide nanoparticles.热休克蛋白 70 和 90 基因的分子特征及其在呼吸空气的湄公河鲶鱼（Clarias magur）暴露于氧化锌纳米颗粒时的表达分析。
Fish Physiol Biochem. 2024 Dec;50(6):2389-2406. doi: 10.1007/s10695-024-01397-4. Epub 2024 Aug 24.
7
The Role of Heat Shock Protein 70 (HSP70) in the Pathogenesis of Ocular Diseases-Current Literature Review.热休克蛋白70（HSP70）在眼部疾病发病机制中的作用——当前文献综述
J Clin Med. 2024 Jun 30;13(13):3851. doi: 10.3390/jcm13133851.
8
Structural insights into GrpEL1-mediated nucleotide and substrate release of human mitochondrial Hsp70.对人线粒体Hsp70的GrpEL1介导的核苷酸和底物释放的结构见解。
bioRxiv. 2024 May 13:2024.05.10.593630. doi: 10.1101/2024.05.10.593630.
9
GDSL Lipase Gene Negatively Regulates Heat Tolerance in Rice Seedlings by Regulating Reactive Oxygen Species Accumulation.GDSL脂肪酶基因通过调控活性氧积累对水稻幼苗耐热性起负调控作用。
Antioxidants (Basel). 2024 May 11;13(5):592. doi: 10.3390/antiox13050592.
10
A review on oligomeric polydispersity and oligomers-dependent holding chaperone activity of the small heat-shock protein IbpB of Escherichia coli.关于大肠杆菌小分子热休克蛋白 IbpB 的低聚物多分散性和低聚物依赖性结合伴侣活性的综述。
Cell Stress Chaperones. 2023 Nov;28(6):689-696. doi: 10.1007/s12192-023-01392-3. Epub 2023 Nov 1.
本文引用的文献
1
Identification of a consensus motif in substrates bound by a Type I Hsp40.鉴定I型热休克蛋白40（Hsp40）所结合底物中的共有基序。
Proc Natl Acad Sci U S A. 2009 Jul 7;106(27):11073-8. doi: 10.1073/pnas.0900746106. Epub 2009 Jun 22.
2
Solution conformation of wild-type E. coli Hsp70 (DnaK) chaperone complexed with ADP and substrate.与二磷酸腺苷（ADP）和底物复合的野生型大肠杆菌热休克蛋白70（DnaK）伴侣蛋白的溶液构象
Proc Natl Acad Sci U S A. 2009 May 26;106(21):8471-6. doi: 10.1073/pnas.0903503106. Epub 2009 May 13.
3
The type I Hsp40 Ydj1 utilizes a farnesyl moiety and zinc finger-like region to suppress prion toxicity.I型热休克蛋白40（Hsp40）Ydj1利用法尼基部分和锌指样区域来抑制朊病毒毒性。
J Biol Chem. 2009 Feb 6;284(6):3628-39. doi: 10.1074/jbc.M807369200. Epub 2008 Dec 4.
4
Structural basis of nucleotide exchange and client binding by the Hsp70 cochaperone Bag2.热休克蛋白70（Hsp70）辅助伴侣蛋白Bag2进行核苷酸交换和与客户蛋白结合的结构基础。
Nat Struct Mol Biol. 2008 Dec;15(12):1309-17. doi: 10.1038/nsmb.1518. Epub 2008 Nov 23.
5
Insights into the structural dynamics of the Hsp110-Hsp70 interaction reveal the mechanism for nucleotide exchange activity.对Hsp110-Hsp70相互作用结构动力学的深入了解揭示了核苷酸交换活性的机制。
Proc Natl Acad Sci U S A. 2008 Oct 28;105(43):16519-24. doi: 10.1073/pnas.0804187105. Epub 2008 Oct 23.
6
Regulated association of misfolded endoplasmic reticulum lumenal proteins with P58/DNAJc3.错误折叠的内质网腔蛋白与P58/DNAJc3的调控性结合
EMBO J. 2008 Nov 5;27(21):2862-72. doi: 10.1038/emboj.2008.199. Epub 2008 Oct 16.
7
Regulated release of ERdj3 from unfolded proteins by BiP.BiP介导未折叠蛋白对ERdj3的调控释放。
EMBO J. 2008 Nov 5;27(21):2873-82. doi: 10.1038/emboj.2008.207. Epub 2008 Oct 16.
8
Farnesylation of Ydj1 is required for in vivo interaction with Hsp90 client proteins.Ydj1的法尼基化是其在体内与Hsp90客户蛋白相互作用所必需的。
Mol Biol Cell. 2008 Dec;19(12):5249-58. doi: 10.1091/mbc.e08-04-0435. Epub 2008 Oct 1.
9
Conserved central domains control the quaternary structure of type I and type II Hsp40 molecular chaperones.保守的中央结构域控制着I型和II型Hsp40分子伴侣的四级结构。
J Mol Biol. 2008 Oct 31;383(1):155-66. doi: 10.1016/j.jmb.2008.08.019. Epub 2008 Aug 14.
10
Functional divergence between co-chaperones of Hsc70.Hsc70共伴侣蛋白之间的功能差异
J Biol Chem. 2008 Oct 3;283(40):27100-9. doi: 10.1074/jbc.M803923200. Epub 2008 Aug 6.
Zotero 插件