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在人和其他灵长类细胞系中检测EB病毒、乙肝病毒、丙肝病毒、人类免疫缺陷病毒1型、人类嗜T淋巴细胞病毒I型和II型以及猿猴泡沫病毒。

Detection of EBV, HBV, HCV, HIV-1, HTLV-I and -II, and SMRV in human and other primate cell lines.

作者信息

Uphoff Cord C, Denkmann Sabine A, Steube Klaus G, Drexler Hans G

机构信息

Department of Human and Animal Cell Lines, German Collection of Microorganisms and Cell Cultures DSMZ, Inhoffenstrasse 7b, 38124 Braunschweig, Germany.

出版信息

J Biomed Biotechnol. 2010;2010:904767. doi: 10.1155/2010/904767. Epub 2010 Apr 29.

DOI:10.1155/2010/904767
PMID:20454443
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2861168/
Abstract

The high prevalence of contaminated cell cultures suggests that viral contaminations might be distributed among cultures. We investigated more than 460 primate cell lines for Epstein-Barr (EBV), hepatitis B (HBV), hepatitis C (HCV), human immunodeficiency virus type 1 (HIV-1), human T-cell leukemia/lymphoma virus I and II (HTLV-I/-II), and squirrel monkey retrovirus (SMRV) infections for risk assessment. None of the cell lines were infected with HCV, HIV-1, or HTLV-I/-II. However, one cell line displayed reverse transcriptase activity. Thirty-nine cell lines harbored EBV DNA sequences. Studies on the lytic phase of EBV revealed that five cell lines produce EBV particles and six further cell lines produced EBV upon stimulation. One cell line contained an integrated HBV genome fragment but showed no virus production. Six cell lines were SMRV-infected. Newly established cell lines should be tested for EBV infections to detect B-lymphoblastoid cell lines (B-LCL). B-LCLs established with EBV from cell line B95-8 should be tested for SMRV infections.

摘要

受污染细胞培养物的高流行率表明病毒污染可能在不同培养物中传播。我们对460多种灵长类细胞系进行了爱泼斯坦-巴尔病毒（EBV）、乙型肝炎病毒（HBV）、丙型肝炎病毒（HCV）、1型人类免疫缺陷病毒（HIV-1）、人类T细胞白血病/淋巴瘤病毒I和II（HTLV-I/-II）以及松鼠猴逆转录病毒（SMRV）感染情况的调查，以进行风险评估。没有细胞系感染HCV、HIV-1或HTLV-I/-II。然而，有一个细胞系显示出逆转录酶活性。39个细胞系含有EBV DNA序列。对EBV裂解期的研究表明，5个细胞系产生EBV颗粒，另外6个细胞系在受到刺激后产生EBV。一个细胞系含有整合的HBV基因组片段，但未显示出病毒产生。6个细胞系感染了SMRV。新建立的细胞系应检测是否感染EBV，以检测B淋巴母细胞系（B-LCL）。用来自B95-8细胞系的EBV建立的B-LCL应检测是否感染SMRV。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e953/2861168/c9c779180c12/JBB2010-904767.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e953/2861168/627cc3b4a4a0/JBB2010-904767.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e953/2861168/c66cdca7a83d/JBB2010-904767.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e953/2861168/08e58954c2d2/JBB2010-904767.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e953/2861168/d1ab19ef171b/JBB2010-904767.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e953/2861168/51954039bf0f/JBB2010-904767.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e953/2861168/43e4f8edcfc9/JBB2010-904767.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e953/2861168/c9c779180c12/JBB2010-904767.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e953/2861168/627cc3b4a4a0/JBB2010-904767.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e953/2861168/c66cdca7a83d/JBB2010-904767.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e953/2861168/08e58954c2d2/JBB2010-904767.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e953/2861168/d1ab19ef171b/JBB2010-904767.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e953/2861168/51954039bf0f/JBB2010-904767.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e953/2861168/43e4f8edcfc9/JBB2010-904767.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e953/2861168/c9c779180c12/JBB2010-904767.007.jpg

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