Al-Attar Atef M
Department of Biological Sciences, Faculty of Sciences, King Abdul Aziz University, P.O. Box 139109, Jeddah 21323, Saudi Arabia.
J Biomed Biotechnol. 2010;2010:203503. doi: 10.1155/2010/203503. Epub 2010 May 5.
The present study was designed to evaluate the influence of alpha-lipoic acid treatment in rats exposed to malathion. Forty adult male rats were used in this study and distributed into four groups. Animals of group 1 were untreated and served as control. Rats of group 2 were orally given malathion at a dose level of 100 mg/kg body weight (BW) for a period of one month. Experimental animals of group 3 were orally given alpha-lipoic acid at a dose level of 20 mg/kg BW and after 3 hours exposed to malathion at the same dose given to group 2. Rats of group 4 were supplemented with alpha-lipoic acid at the same dose given to group 3. The activities of serum glutamic oxaloacetic acid transaminase (GOT), glutamic pyruvic acid transaminase (GPT), alkaline phosphatase (ALP), and acid phosphatase (ACP), and the values of creatinine, urea, and uric acid were statistically increased, while the values of total protein and total albumin were significantly decreased in rats exposed to malathion. Moreover, administration of malathion for one month resulted in damage of liver and kidney structures. Administration of alpha-lipoic acid before malathion exposure to rat can prevent severe alterations of hemato-biochemical parameters and disruptions of liver and kidney structures. In conclusion, this study obviously demonstrated that pretreatment with alpha-lipoic acid significantly attenuated the physiological and histopathological alterations induced by malathion. Also, the present study identifies new areas of research for development of better therapeutic agents for liver, kidney, and other organs' dysfunctions and diseases.
本研究旨在评估α-硫辛酸处理对暴露于马拉硫磷的大鼠的影响。本研究使用了40只成年雄性大鼠，并将其分为四组。第1组动物未接受处理，作为对照组。第2组大鼠口服给予剂量为100 mg/kg体重（BW）的马拉硫磷，持续1个月。第3组实验动物口服给予剂量为20 mg/kg BW的α-硫辛酸，3小时后以与第2组相同的剂量暴露于马拉硫磷。第4组大鼠补充与第3组相同剂量的α-硫辛酸。暴露于马拉硫磷的大鼠血清谷氨酸草酰乙酸转氨酶（GOT）、谷氨酸丙酮酸转氨酶（GPT）、碱性磷酸酶（ALP）和酸性磷酸酶（ACP）的活性以及肌酐、尿素和尿酸的值在统计学上有所增加，而总蛋白和总白蛋白的值则显著降低。此外，给予马拉硫磷1个月会导致肝脏和肾脏结构受损。在大鼠接触马拉硫磷之前给予α-硫辛酸可以预防血液生化参数的严重改变以及肝脏和肾脏结构的破坏。总之，本研究明显表明，用α-硫辛酸预处理可显著减轻马拉硫磷诱导的生理和组织病理学改变。此外，本研究确定了开发用于治疗肝脏、肾脏和其他器官功能障碍及疾病的更好治疗药物的新研究领域。
