Shih Po-Chen, Kuo Chia-Hao, Juang Jenh-Yih, Liu Cheng-Hsien, Hsu Long, Liu Cheng-Tzu
Department of Electrophysics, National Chiao Tung University, Hsinchu 300, Taiwan.
J Biomed Biotechnol. 2010;2010:319059. doi: 10.1155/2010/319059. Epub 2010 May 3.
We have designed and fabricated a novel chemotactic gradient Labchip for studying cell migration quantitatively. Owing to the great potential of garlic and its preparations in developing antiinflammatory drugs, the aim of the present study is to investigate the effect of garlic oil on the locomotion of a neutrophil-like cell by measuring the dynamic features of cell migration including migration direction, average migration speed, chemotactic index (CI), and motility index (MI) with the newly designed Labchip. We found that garlic oil treatment lowered the values of CI and MI and reduced the average speed of cell migration from 13 to 8 microm/min. The results indicate that garlic oil is a potential inhibitor for neutrophil-like cell migration and chemotactic responsiveness. By comparing with the effects of nocodazole and cytochalasin B, we also suggest that the antiinflammatory activity exhibited by garlic oil was mainly through inhibiting the assembly-disassembly processes of the cytoskeleton.
我们设计并制造了一种新型趋化梯度微流控芯片，用于定量研究细胞迁移。鉴于大蒜及其制剂在开发抗炎药物方面的巨大潜力，本研究的目的是通过使用新设计的微流控芯片测量细胞迁移的动态特征，包括迁移方向、平均迁移速度、趋化指数（CI）和运动指数（MI），来研究大蒜油对类中性粒细胞运动的影响。我们发现，大蒜油处理降低了CI和MI的值，并将细胞迁移的平均速度从13微米/分钟降低到8微米/分钟。结果表明，大蒜油是类中性粒细胞迁移和趋化反应的潜在抑制剂。通过与诺考达唑和细胞松弛素B的作用进行比较，我们还表明大蒜油表现出的抗炎活性主要是通过抑制细胞骨架的组装-拆卸过程。
