College of Veterinary Medicine (BK21 Plus Project Team), Chonnam National University, 27 Yongbong-ro, Buk-gu, Gwangju 61186, Korea.
Namhae Garlic Research Institute, 2465-8 Namhae-daero, Namhae-gun, Gyungnam 52430, Korea.
Nutrients. 2018 Nov 4;10(11):1659. doi: 10.3390/nu10111659.
Garlic () has traditionally been used as a medicinal food and exhibits various beneficial activities, such as antitumor, antimicrobial, hypolipidemic, antiarthritic, and hypoglycemic activities. The aim of this study was to explore the preventive effect of garlic oil (GO) and its organosulfur component diallyl disulfide (DADS) on cigarette smoke (CS)-induced airway inflammation. Mice were exposed to CS daily for 1 h (equivalent to eight cigarettes per day) for two weeks, and intranasally instilled with lipopolysaccharide (LPS) on day 12 after the initiation of CS exposure. GO and DADS were administered to mice by oral gavage, both at rates of 20 and 40 mg/kg, for 1 h before CS exposure for two weeks. In the bronchoalveolar lavage fluid, GO and DADS inhibited the elevation in the counts of inflammatory cells, particularly neutrophils, which were induced in the CS and LPS (CS + LPS) group. This was accompanied by the lowered production (relative to the CS + LPS group) of interleukin (IL)-1β, IL-6, and tumor necrosis factor-α. Histologically, GO and DADS inhibited the CS- and LPS-induced infiltration of inflammatory cells into lung tissues. Additionally, GO and DADS inhibited the phosphorylation of extracellular signal-regulated kinase and the expression of matrix metalloproteinase-9 in the lung tissues. Taken together, these findings indicate that GO and DADS could be a potential preventive agent in CS-induced airway inflammation.
大蒜 () 传统上被用作药用食品，具有多种有益的活性，如抗肿瘤、抗菌、降血脂、抗关节炎和降血糖活性。本研究旨在探讨大蒜油 (GO) 和其有机硫成分二烯丙基二硫 (DADS) 对香烟烟雾 (CS) 诱导的气道炎症的预防作用。小鼠每天暴露于 CS 中 1 小时（相当于每天 8 支香烟），在 CS 暴露开始后第 12 天经鼻腔内滴注脂多糖 (LPS)。GO 和 DADS 通过灌胃给药，在 CS 暴露前 1 小时内以 20 和 40mg/kg 的剂量给药，连续两周。在支气管肺泡灌洗液中，GO 和 DADS 抑制了 CS 和 LPS（CS + LPS）组中诱导的炎症细胞（特别是中性粒细胞）计数的升高。这伴随着白细胞介素 (IL)-1β、IL-6 和肿瘤坏死因子-α产生的降低（相对于 CS + LPS 组）。组织学上，GO 和 DADS 抑制了 CS 和 LPS 诱导的炎性细胞浸润到肺组织中。此外，GO 和 DADS 抑制了肺组织中细胞外信号调节激酶的磷酸化和基质金属蛋白酶-9 的表达。综上所述，这些发现表明 GO 和 DADS 可能是 CS 诱导的气道炎症的潜在预防剂。
