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用于临床应用的原代人成纤维细胞和角质形成细胞中衰老或永生化诱导的蛋白质组改变的比较定量分析。

Comparative quantitation of proteome alterations induced by aging or immortalization in primary human fibroblasts and keratinocytes for clinical applications.

作者信息

Sprenger Adrian, Küttner Victoria, Biniossek Martin L, Gretzmeier Christine, Boerries Melanie, Mack Claudia, Has Cristina, Bruckner-Tuderman Leena, Dengjel Jörn

机构信息

Freiburg Institute for Advanced Studies, School of Life Science-LIFENET, University of Freiburg, Albertstr. 19, 79104 Freiburg, Germany.

出版信息

Mol Biosyst. 2010 Sep;6(9):1579-82. doi: 10.1039/c003962d. Epub 2010 May 10.

DOI:10.1039/c003962d
PMID:20454738
Abstract

We monitor proteome changes of primary human skin fibroblasts and keratinocytes during cell culture and compare them to respective immortalized cell lines using stable isotope labeling by amino acids to address their suitability as cell models for clinical disease proteomics.

摘要

我们监测原代人皮肤成纤维细胞和角质形成细胞在细胞培养过程中的蛋白质组变化，并通过氨基酸稳定同位素标记将它们与各自的永生化细胞系进行比较，以评估它们作为临床疾病蛋白质组学细胞模型的适用性。

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