Research Center skinitial, Department of Dermatology, Medical Center, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.
Flavex Naturextrakte GmbH, 66780 Rehlingen, Germany.
Biomolecules. 2021 Mar 2;11(3):371. doi: 10.3390/biom11030371.
Psoriasis is a chronic inflammatory skin disease characterized by hyperproliferation of keratinocytes and expression of pro-inflammatory cytokines in the epidermis. New biological drugs were developed for the systemic treatment of moderate to severe psoriasis. However, products for the topical treatment of mild psoriasis are still required. Here, we examined the effect of natural compounds on psoriasis-like keratinocytes in vitro and ex vivo. Psoriasis-like keratinocytes were generated by treating human primary keratinocytes with the psoriasis-associated cytokines IL-17A, TNF-α and IL-22. Initially, 10 botanical extracts from Ayurvedic Medicine, Traditional Chinese Medicine, Northern American traditional medicine and Occidental Monastic Medicine were investigated using BrdU assays and IL-6 and IL-8 ELISAs. , and turned out to be the most effective plant extracts. In vitro, the plant extracts inhibited the expression of anti-microbial peptides (β-defensin 2), the hyperproliferation marker keratin 17, the glucose transporter 1 and downregulated the nuclear translocation of NF-κB and pSTAT3. In an ex vivo psoriasis model, displayed the most prominent anti-proliferative and anti-inflammatory effect. In conclusion, among the plant extracts investigated, showed the most promising anti-psoriatic effect. It is an interesting candidate for topical psoriasis treatment that should be further studied in clinical trials.
银屑病是一种慢性炎症性皮肤病，其特征是角质形成细胞过度增殖和表皮中促炎细胞因子的表达。新的生物药物被开发用于中重度银屑病的系统治疗。然而，仍需要用于轻度银屑病的局部治疗产品。在这里，我们研究了天然化合物对体外和体内银屑病样角质形成细胞的影响。通过用与银屑病相关的细胞因子 IL-17A、TNF-α 和 IL-22 处理人原代角质形成细胞，产生了银屑病样角质形成细胞。最初，使用 BrdU 测定法和 IL-6 和 IL-8 ELISA 研究了来自阿育吠陀医学、中药、北美传统医学和西方僧侣医学的 10 种植物提取物。结果表明，和 是最有效的植物提取物。在体外，植物提取物抑制了抗菌肽（β-防御素 2）、过度增殖标志物角质形成细胞 17、葡萄糖转运蛋白 1 的表达，并下调了 NF-κB 和 pSTAT3 的核易位。在体外银屑病模型中， 表现出最显著的抗增殖和抗炎作用。总之，在所研究的植物提取物中， 表现出最有希望的抗银屑病作用。它是一种用于局部治疗银屑病的有趣候选药物，应在临床试验中进一步研究。
