Fenby Nick, Pu Hong, Pennell Roger, Praekelt Uta, Day Rob, Scott Rod
Department of Biology and Biochemistry, Bath University, Bath BA2 7AY, UK.
Sex Plant Reprod. 2010 Dec;23(4):255-64. doi: 10.1007/s00497-010-0142-z. Epub 2010 May 8.
Simple de novo screens in Arabidopsis thaliana have previously identified mutants that affect endosperm development but viable-embryo mutants have not been identified. Our strategy to identify autonomous embryo development was to uncouple embryo and endosperm fertilisation. This involved a male-sterile mutant population being crossed with a distinct pollen parent--the pollen was needed to initiate endosperm development and because it was distinct, the maternal progeny could be selected from the hybrid population. This process was refined over three stages, resulting in a viable approach to screen for autonomous embryo mutants. From 8,000 screened plants, a mutation was isolated in which the integument cells extended from the ovule and proliferated into a second complete twinned ovule. Some embryos from the mutant were normal but others developed fused cotyledons. In addition, a proportion of the progeny lacked paternal genes.
此前在拟南芥中进行的简单从头筛选已鉴定出影响胚乳发育的突变体，但尚未鉴定出有活力胚的突变体。我们鉴定自主胚胎发育的策略是使胚胎和胚乳受精解偶联。这涉及将一个雄性不育突变体群体与一个不同的花粉亲本杂交——需要花粉来启动胚乳发育，并且由于花粉不同，母本后代可以从杂交群体中筛选出来。这个过程分三个阶段进行了优化，形成了一种筛选自主胚胎突变体的可行方法。在8000株被筛选的植株中，分离出一个突变体，其珠被细胞从胚珠延伸并增殖形成第二个完整的孪生胚珠。该突变体的一些胚胎是正常的，但其他胚胎发育出融合的子叶。此外，一部分后代缺乏父本基因。
