Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, University of Paris-Saclay, Route de St-Cyr (RD10), 78026, Versailles Cedex, France.
École Doctorale 567 Sciences du Végétal, University Paris-Sud, University of Paris-Saclay, bat 360, 91405, Orsay Cedex, France.
BMC Plant Biol. 2019 Jul 10;19(1):304. doi: 10.1186/s12870-019-1877-9.
In flowering plants, proper seed development is achieved through the constant interplay of fertilization products, embryo and endosperm, and maternal tissues. Communication between these compartments is supposed to be tightly regulated at their interfaces. Here, we characterize the deposition pattern of an apoplastic lipid barrier between the maternal inner integument and fertilization products in Arabidopsis thaliana seeds.
We demonstrate that an apoplastic lipid barrier is first deposited by the ovule inner integument and undergoes de novo cutin deposition following central cell fertilization and relief of the FERTILIZATION INDEPENDENT SEED Polycomb group repressive mechanism. In addition, we show that the WIP zinc-finger TRANSPARENT TESTA 1 and the MADS-Box TRANSPARENT TESTA 16 transcription factors act maternally to promote its deposition by regulating cuticle biosynthetic pathways. Finally, mutant analyses indicate that this apoplastic barrier allows correct embryo sliding along the seed coat.
Our results revealed that the deposition of a cutin apoplastic barrier between seed maternal and zygotic tissues is part of the seed coat developmental program.
在开花植物中，通过受精产物、胚胎和胚乳以及母体组织的不断相互作用，才能实现正常的种子发育。这些隔室之间的通讯应该在它们的界面处受到严格的调控。在这里，我们描述了拟南芥种子中母体内珠被与受精产物之间质外体脂质屏障的沉积模式。
我们证明，质外体脂质屏障首先由胚珠内珠被沉积，并在中央细胞受精后和 FERTILIZATION INDEPENDENT SEED Polycomb 组抑制机制解除后，经历新的角质层沉积。此外，我们还表明，WIP 锌指 TRANSPARENT TESTA 1 和 MADS-Box TRANSPARENT TESTA 16 转录因子通过调节角质层生物合成途径，以母体方式发挥作用，促进其沉积。最后，突变分析表明，这种质外体屏障允许胚胎沿着种皮正确滑动。
我们的结果表明，在种子母体和合子组织之间沉积一层角质质外体屏障是种皮发育程序的一部分。
