Hebei Agriculture University, Qinhuangdao, China.
Biochem Genet. 2010 Jun;48(5-6):504-15. doi: 10.1007/s10528-010-9334-3. Epub 2010 Feb 21.
The p8 gene encodes a transcription factor known to modulate cell growth, division, and apoptosis and influences gene expression. In this study, an Atlantic halibut (Hippoglossus hippoglossus) homolog of the p8 gene was cloned, sequenced, and characterized. The full-length p8 cDNA consists of 601 bp and encodes 76 amino acids with a molecular mass of 9 kD. The bHLH region is well conserved between Atlantic halibut and other animals. Analysis by RT-PCR showed that the p8 transcript is constitutively expressed in 9 of the 12 tissues tested: pancreas, intestine, stomach, gill, head kidney, heart, liver, ovary, and spleen. A predicted microRNA target site was found in the 3'UTR of Atlantic halibut p8 mRNA. We speculate that the target site may pair to microRNA molecules because the target site resides in a big loop, a space large enough for the binding of microRNA molecules.
p8 基因编码一种已知调节细胞生长、分裂和凋亡并影响基因表达的转录因子。在这项研究中，克隆、测序并表征了大西洋比目鱼（Hippoglossus hippoglossus）的 p8 基因同源物。全长 p8 cDNA 由 601bp 组成，编码 76 个氨基酸，分子量为 9kD。bHLH 区域在大西洋比目鱼和其他动物之间高度保守。通过 RT-PCR 分析表明，p8 转录本在 12 种检测组织中的 9 种中持续表达：胰腺、肠、胃、鳃、头肾、心脏、肝脏、卵巢和脾脏。在大西洋比目鱼 p8 mRNA 的 3'UTR 中发现了一个预测的 microRNA 靶位点。我们推测靶位点可能与 microRNA 分子配对，因为靶位点位于一个大环中，这个空间足够容纳 microRNA 分子的结合。
