L-蛋氨酸诱导 MCF-7 和 LNCaP 癌细胞分子特征的改变。
L-methionine-induced alterations in molecular signatures in MCF-7 and LNCaP cancer cells.
机构信息
Department of Pathology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA,
出版信息
J Cancer Res Clin Oncol. 2011 Mar;137(3):441-53. doi: 10.1007/s00432-010-0897-5. Epub 2010 May 9.
BACKGROUND
Methionine inhibits proliferation of breast and prostate cancer cells. Here, we determined the influence of L-methionine on functional molecular signatures in these cell lines.
METHODS
MCF-7 and LNCaP cells were treated with L-methionine (5 mg/ml) for 72 h. Changes in molecular signatures of these cells were examined by microarray analysis of 15,814 probes in triplicate experiments.
RESULTS
In LNCaP cells, 325 genes were up-regulated by methionine, and 517 genes down-regulated. In MCF-7 cells, 86 genes were up-regulated and 135 genes down-regulated. Ninety-eight genes were regulated in the same direction by methionine in both cells lines, and five other genes were changed in expression in opposite directions.
CONCLUSION
Several of the up-regulated genes encode proteins involved in cellular redox regulation, suggesting that methionine may enhance antioxidant mechanisms. Many of the down-regulated genes belong to protein kinase families that may be related to the anti-proliferative effects of methionine on breast and prostate cancer cells.
背景
蛋氨酸可抑制乳腺癌和前列腺癌细胞的增殖。在此，我们研究了 L-蛋氨酸对这些细胞系中功能性分子特征的影响。
方法
用 L-蛋氨酸（5mg/ml）处理 MCF-7 和 LNCaP 细胞 72 小时。通过对这三种细胞系的 15814 个探针的微阵列分析，检测这些细胞的分子特征变化。
结果
在 LNCaP 细胞中，有 325 个基因被蛋氨酸上调，517 个基因被下调。在 MCF-7 细胞中，有 86 个基因上调，135 个基因下调。有 98 个基因在这两种细胞系中被蛋氨酸以相同的方向调控，另外 5 个基因则以相反的方向表达。
结论
一些上调的基因编码参与细胞氧化还原调节的蛋白质，表明蛋氨酸可能增强抗氧化机制。许多下调的基因属于蛋白激酶家族，可能与蛋氨酸对乳腺癌和前列腺癌细胞的抗增殖作用有关。
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