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亚硒酸盐(SeIV)和单质硒(Se0)对芽孢杆菌解毒能力的比较评估

Comparative assessment of selenite (SeIV) detoxification to elemental selenium (Se0) by Bacillus sp.

机构信息

Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore 54590, Pakistan.

出版信息

Biotechnol Lett. 2010 Sep;32(9):1255-9. doi: 10.1007/s10529-010-0291-z. Epub 2010 May 9.

Abstract

Bacillus licheniformis, B. subtilis, B. cereus, Bacillus pumilus and Exiguobacterium sp., which were resistant up to 20 mg Na(2)SeO(3)/ml in nutrient broth and 40 mg/ml on nutrient agar plates, were isolated from contaminated soil and water. They grew from 25 to 45 degrees C and pH 5 to 9. They had multiple metal and antibiotic resistances. All strains reduced selenite (SeIV) to elemental selenium (Se0) aerobically with a maximum reduction of 97% by B. pumilus after 144 h with Na(2)SeO(3) at 500 microg/ml.

摘要

地衣芽孢杆菌、枯草芽孢杆菌、蜡样芽孢杆菌、短小芽孢杆菌和极端小球菌,在营养肉汤中可耐受高达 20mg/ml 的亚硒酸钠,在营养琼脂平板上可耐受高达 40mg/ml,它们从污染的土壤和水中分离得到。它们在 25 到 45 摄氏度和 pH 值 5 到 9 的条件下生长。它们具有多种金属和抗生素抗性。所有菌株都可以将亚硒酸盐(SeIV)在有氧条件下还原为元素硒(Se0),其中短小芽孢杆菌在 500μg/ml 的亚硒酸钠作用下,144 小时后最大还原率达到 97%。

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