Institute for Applied Botany and Pharmacognosy, University of Veterinary Medicine, Vienna, Austria.
Planta Med. 2010 Nov;76(16):1934-7. doi: 10.1055/s-0030-1249908. Epub 2010 May 7.
Hellbori nigri rhizoma is a drug that is difficult to distinguish from other species of the genus Helleborus. In this communication we present a DNA-based identification by high-resolution melting analysis (HRM) that is able to differentiate between Helleborus niger and other species of the genus. HRM is a very specific, time- and labour-saving method for identifying DNA sequence variations and is ideally suitable for routine PCR analysis. The HRM assay developed is specific for the genus Helleborus. This method not only detects the presence of the target species H. niger but also, to a certain extent, identifies other Helleborus species by their different melting curve shapes. Markers were developed based on the trnL-trnF intergenic spacer and on the matK sequence. For an unambiguous identification of Helleborus niger, melting curves of both markers should be used.
黑藜芦根茎是一种难以与其他嚏根草属物种区分的药物。在本通讯中，我们提出了一种基于 DNA 的高分辨率熔解分析 (HRM) 鉴定方法，能够区分黑藜芦和该属的其他物种。HRM 是一种非常特异、省时省力的方法，可用于鉴定 DNA 序列变异，非常适合常规 PCR 分析。开发的 HRM 检测方法特异性针对嚏根草属。该方法不仅可以检测目标物种黑藜芦的存在，而且还可以通过不同的熔解曲线形状在一定程度上识别其他嚏根草属物种。标记物是基于 trnL-trnF 基因间隔区和 matK 序列开发的。为了明确鉴定黑藜芦，应使用这两个标记物的熔解曲线。
