Berisha Naim, Millaku Fadil, Gashi Bekim, Krasniqi Elez, Novak Johannes
Department of Biology, Faculty of Mathematics and Natural Sciences, University of Prishtina "Hasan Prishtina", Mother Teresa, str., n.n., 10 000 Prishtina, Republic of Kosovo ; Institute of Animal Nutrition and Functional Plant Compounds, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria.
Department of Biology, Faculty of Mathematics and Natural Sciences, University of Prishtina "Hasan Prishtina", Mother Teresa, str., n.n., 10 000 Prishtina, Republic of Kosovo.
Physiol Mol Biol Plants. 2015 Jan;21(1):117-22. doi: 10.1007/s12298-014-0275-x. Epub 2014 Dec 31.
Primula veris L. (Primulaceae) is a long lived perennial and well known pharmaceutical plant, widely collected for these reasons in almost all SE Europe and particularly in Kosovo. The aim of the study is to determine molecular polymorphism of cowslip (P. veris L.) populations from Kosovo. DNA extracted from leaves were investigated in details for presence of polymorphism. RAPD analyses were conducted using 20 different short primers. Genomic DNA amplification profiles were analyzed and processed using data labelling. Comparison between cowslip populations in genetic composition revealed that samples from Bogaj were too distinct on their own. Molecular variation was observed to be more within populations (73 %) as compared to among populations (27 %). On the other hand, genetic distance of populations revealed that the highest genetic distance is between Leqinat and Maja e Madhe. Mean values of expected heterozygosity were highest in Bogaj population, while lowest in Maja e Madhe population. The obtained results indicated that Bogaj population are more polymorphic. From the obtained data it can be concluded that RAPD markers provided a useful technique to study genetic diversity in P. veris L. populations. This technology allows identification and assessment of the genetic similarities and differences among plant populations.
报春花（报春花科）是一种多年生长寿且著名的药用植物，由于这些原因，在几乎整个东南欧，尤其是在科索沃被广泛采集。本研究的目的是确定科索沃报春花（Primula veris L.）种群的分子多态性。对从叶片中提取的DNA进行详细研究，以检测多态性的存在。使用20种不同的短引物进行随机扩增多态性DNA（RAPD）分析。使用数据标记对基因组DNA扩增图谱进行分析和处理。报春花种群之间遗传组成的比较表明，来自博加伊的样本本身差异很大。观察到分子变异在种群内部（73%）比在种群之间（27%）更为明显。另一方面，种群的遗传距离显示，莱基纳特和马贾埃马德之间的遗传距离最大。预期杂合度的平均值在博加伊种群中最高，而在马贾埃马德种群中最低。所得结果表明博加伊种群的多态性更高。从所得数据可以得出结论，RAPD标记为研究报春花种群的遗传多样性提供了一种有用的技术。这项技术可以识别和评估植物种群之间的遗传异同。
