Watts J L, Ray C H, Washburn P J
Mastitis Research Laboratory, Louisiana State University Agricultural Center, Homer 71040.
J Dairy Sci. 1991 Feb;74(2):426-8. doi: 10.3168/jds.S0022-0302(91)78186-1.
The utility of trehalose-mannitol broth and arabinose-cellobiose broth for identification of Staphylococcus epidermidis and novobiocin-resistant staphylococci was determined using 236 coagulase-negative staphylococci isolated from bovine mammary glands. None of the 49 S. epidermidis strains was positive in trehalose-mannitol broth; whereas, all strains of Staphylococcus hyicus, Staphylococcus chromogenes, Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus warneri, and Staphylococcus simulans were positive. Of the novobiocin-resistant staphylococcal species, only Staphylococcus saprophyticus was negative in arabinose-cellobiose broth. Except for one strain of Staphylococcus sciuri and one strain of Staphylococcus kloosii, all remaining strains of novobiocin-resistant staphylococcal species were positive in arabinose-cellobiose broth. Results indicate that trehalose-mannitol broth is an acceptable method for identification of S. epidermidis isolated from bovine mammary glands. Furthermore, arabinose-cellobiose broth is a useful method of screening for novobiocin-resistant staphylococci.
使用从牛乳腺分离出的236株凝固酶阴性葡萄球菌，测定了海藻糖 - 甘露醇肉汤和阿拉伯糖 - 纤维二糖肉汤对表皮葡萄球菌和耐新生霉素葡萄球菌的鉴定效用。49株表皮葡萄球菌菌株在海藻糖 - 甘露醇肉汤中均为阴性；而所有猪葡萄球菌、产色葡萄球菌、溶血葡萄球菌、人葡萄球菌、沃氏葡萄球菌和模仿葡萄球菌菌株均为阳性。在耐新生霉素葡萄球菌物种中，只有腐生葡萄球菌在阿拉伯糖 - 纤维二糖肉汤中为阴性。除1株松鼠葡萄球菌和1株克氏葡萄球菌外，所有其余耐新生霉素葡萄球菌菌株在阿拉伯糖 - 纤维二糖肉汤中均为阳性。结果表明，海藻糖 - 甘露醇肉汤是鉴定从牛乳腺分离出的表皮葡萄球菌的可接受方法。此外，阿拉伯糖 - 纤维二糖肉汤是筛选耐新生霉素葡萄球菌的有用方法。
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