UPMC Univ. Paris 06, The Marine Plants and Biomolecules Laboratory, UMR 7139, Station Biologique de Roscoff, Place Georges Teissier, BP74, 29682 Roscoff Cedex, France.
New Phytol. 2010 Oct;188(1):42-51. doi: 10.1111/j.1469-8137.2010.03273.x. Epub 2010 Apr 28.
• Ectocarpus siliculosus has been proposed as a genetic and genomic model for the brown algae and the 214 Mbp genome of this organism has been sequenced. The aim of this project was to obtain a chromosome-scale view of the genome by constructing a genetic map using microsatellite markers that were designed based on the sequence supercontigs. • To map genetic markers, a segregating F(2) population was generated from a cross between the sequenced strain (Ec 32) and a compatible strain from northern Chile. Amplified fragment length polymorphism (AFLP) analysis indicated a significant degree of polymorphism (41%) between the genomes of these two parental strains. Of 1,152 microsatellite markers that were selected for analysis based on their location on long supercontigs, their potential as markers and their predicted ability to amplify a single genomic locus, 407 were found to be polymorphic. • A genetic map was constructed using 406 markers, resulting in 34 linkage groups. The 406 markers anchor 325 of the longest supercontigs on to the map, representing 70.1% of the genome sequence. • The Ectocarpus genetic map described here not only provides a large-scale assembly of the genome sequence, but also represents an important tool for future genetic analysis using this organism.
• 泡叶藻已被提议作为褐藻的遗传和基因组模型，并且该生物体的 214 Mbp 基因组已被测序。本项目的目的是通过构建基于序列超contigs 设计的微卫星标记的遗传图谱，获得基因组的染色体规模图谱。 • 为了绘制遗传标记，从测序菌株 (Ec 32) 和来自智利北部的相容菌株之间的杂交后代中产生了一个分离的 F(2)群体。扩增片段长度多态性 (AFLP) 分析表明，这两个亲本菌株的基因组之间存在显著程度的多态性 (41%)。基于其在长超contigs 上的位置、作为标记的潜力及其预测能够扩增单个基因组位点的能力，从 1152 个微卫星标记中选择了 407 个进行分析，发现它们是多态的。 • 使用 406 个标记构建了遗传图谱，得到了 34 个连锁群。这 406 个标记将最长的 325 个超contigs 锚定在图谱上，代表基因组序列的 70.1%。 • 这里描述的泡叶藻遗传图谱不仅提供了基因组序列的大规模组装，而且还代表了使用该生物体进行未来遗传分析的重要工具。
