Marine Biological Association, Citadel Hill, Plymouth PL1 2PB, UK.
New Phytol. 2010 Oct;188(1):30-41. doi: 10.1111/j.1469-8137.2010.03303.x. Epub 2010 Jun 1.
• The origin of the Ectocarpus strain used for genome sequencing (the 'genome strain') was Peru, where no Ectocarpus had been reported previously. To study the genetic diversity in the region and to increase the number of individuals from this area available for genetic experiments, 119 new Ectocarpus strains were isolated from eight localities along the 3000 km of coastline from central Peru to central Chile. • Internal transcribed spacer 1 (ITS1) genotyping revealed nine different genotypes, five of which were endemic to the area studied and three of which were previously unknown. • Individuals of the same genotype as the genome strain occurred from Peru to northernmost Chile, representing 61% of the samples in this area, from which five more genotypes were isolated. Further south, down to central Chile, most individuals belonged to Ectocarpus siliculosus, Ectocarpus fasciculatus and Ectocarpus crouaniorum. In sexual crosses, the genome strain and the new isolates of the same genotype were fully compatible. • Sequences from four nuclear and cytoplasmic genetic markers (ITS1, ITS2, Rubisco spacer and Cytochrome-c oxidase subunit 3 (cox3)) separated the genome strain from the known species of Ectocarpus. It may in future be recognized as a separate species.
• 用于基因组测序的浒苔品系（“基因组品系”）的起源地是秘鲁，此前那里没有报道过浒苔。为了研究该地区的遗传多样性，并增加该地区可用于遗传实验的个体数量，从秘鲁中部到智利中部的 3000 公里海岸线的 8 个地点分离了 119 个新的浒苔品系。• 内部转录间隔区 1（ITS1）基因分型显示有 9 种不同的基因型，其中 5 种是该地区特有的，3 种是以前未知的。• 与基因组品系相同基因型的个体从秘鲁到智利最北部都有出现，代表了该地区 61%的样本，从中又分离出了另外 5 种基因型。再往南，到智利中部，大多数个体属于石莼、石莼和石莼。在有性杂交中，基因组品系和相同基因型的新分离株完全兼容。• 来自四个核和细胞质遗传标记（ITS1、ITS2、Rubisco 间隔区和细胞色素 c 氧化酶亚基 3（cox3）的序列将基因组品系与已知的浒苔物种区分开来。它将来可能被认定为一个独立的物种。
