Clinical Medical Research Center, the Second Clinical Medical College (Shenzhen People's Hospital) Ji'nan University, Shenzhen, China.
Clin Exp Immunol. 2010 Aug;161(2):357-63. doi: 10.1111/j.1365-2249.2010.04178.x. Epub 2010 May 7.
Allogeneic pancreatic islet transplantation theoretically represents a cure for type 1 diabetes. However, current immune suppressive therapies are often associated with undesired side effects. Given this problem, and the shortage of human islet donors, the majority of type 1 diabetes patients cannot currently be offered an islet transplant. However, it has been found that mesenchymal stem cells (MSCs) could exert unique immunosuppressive effects both in vitro and in vivo. Herein we transplanted allogeneic 200 islets alone or in combination with MSCs (3 x 10(6) cells) under the kidney capsules of diabetic C57LB/6 mouse. We found that the ratios of T helper type 1 (Th1) to Th2 and Tc1 to Tc2 were reduced, and the numbers of naive and memory T cells were down-regulated in peripheral blood after transplantation. In addition, the maturation, endocytosis and interleukin-12 secretion of dendritic cell (DCs)-derived bone marrow cells (BMCs) from receptor mice were suppressed. Rejection reaction was alleviated by MSCs which exerted suppressive effects through T lymphocyte subsets and DCs.
同种异体胰岛移植理论上代表了 1 型糖尿病的一种治愈方法。然而，目前的免疫抑制疗法常常伴有不良的副作用。鉴于这一问题和胰岛供体的短缺，大多数 1 型糖尿病患者目前无法接受胰岛移植。然而，已经发现间充质干细胞 (MSCs) 在体外和体内都能发挥独特的免疫抑制作用。在此，我们将同种异体 200 个胰岛单独或与 MSCs（3 x 10(6) 个细胞）一起移植到糖尿病 C57LB/6 小鼠的肾脏包膜下。我们发现，移植后外周血中辅助性 T 细胞 1 (Th1) 与 Th2 和 Tc1 与 Tc2 的比值降低，幼稚和记忆 T 细胞的数量减少。此外，受体小鼠来源的树突状细胞 (DCs) 骨髓细胞 (BMCs) 的成熟、内吞和白细胞介素-12 分泌受到抑制。MSCs 通过 T 淋巴细胞亚群和 DCs 发挥抑制作用，减轻了排斥反应。
