Lim Jong Yeon, Min Byoung Hoon, Kim Byoung Geun, Shin Jun-Seop, Park Chang Sook, Yoon Tai Wook, Han Sung Sik
Korea Islet Transplantation Institution Inc, School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
Pancreas. 2009 May;38(4):447-53. doi: 10.1097/MPA.0b013e318197a62e.
Recent studies have demonstrated the impressive expansion of beta cells in vitro. But unfortunately, expanded beta cells do not function in the same way as fully differentiated beta cells. Therefore, we developed a condition that would allow islet cells to proliferate while maintaining their endocrine function.
We tested the different use of growth factors in a different culture period. And we tested the possibility of adult islets, which expanded during a short period, as a clinical source of islet cells by comparing the efficiency of transplantation of cultured islets with that of fresh islets.
The islets showed a time-dependent increase in proliferative activity, reaching 32.2% on day 5. After 5 days of culture, the efficiency of transplantation of cultured islets was increased (2-fold) in comparison to that of noncultured islets. Moreover, islet transplantation immediately induced normoglycemia at a level equal to native islets.
These findings suggest that adult beta cells have the potential to proliferate while maintaining their endocrine function, which can be improved through careful regulation of proliferation and differentiation.
最近的研究已证明β细胞在体外能显著扩增。但遗憾的是，扩增后的β细胞与完全分化的β细胞功能不同。因此，我们开发了一种条件，使胰岛细胞能够增殖，同时维持其内分泌功能。
我们在不同的培养时期测试了生长因子的不同使用方式。并且通过比较培养胰岛与新鲜胰岛的移植效率，测试了短期内扩增的成年胰岛作为胰岛细胞临床来源的可能性。
胰岛的增殖活性呈时间依赖性增加，在第5天达到32.2%。培养5天后，与未培养的胰岛相比，培养胰岛的移植效率提高了（2倍）。此外，胰岛移植立即诱导血糖正常，水平与天然胰岛相当。
这些发现表明，成年β细胞有增殖的潜力，同时维持其内分泌功能，通过仔细调控增殖和分化可改善这一功能。
