Barash Mark, Reshef Ayeleth, Brauner Paul
Forensic Biology Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police National Headquarters, Jerusalem, Israel.
J Forensic Sci. 2010 Jul;55(4):1058-64. doi: 10.1111/j.1556-4029.2010.01416.x. Epub 2010 Apr 28.
The selection of the appropriate method of collection of biological material from crime scene items can be crucial to obtaining a DNA profile. The three techniques commonly used for sampling items are: cutting, swabbing, and taping. The tape sampling technique offers an advantage, in that it enables the collection of a potentially highly informative source of DNA, shed epithelial cells, from selected areas on crime scene items (the inside fingers of a glove, for instance). Furthermore, surface collection of biological material by taping reduces co-sampling of known PCR inhibitors such as clothing dyes. The correct choice of tape for crime scene item sampling is important. Not all tapes are suitable for biological trace evidence collection as well as DNA extraction. We report on one tape that met both these criteria. Three different cases are presented which demonstrate the usefulness of adhesive tape sampling of crime items. Finally, the advantages of the tape collection technique are discussed and guidelines for preferred areas of tape sampling on various casework items are presented.
从犯罪现场物品中选择合适的生物材料采集方法对于获取DNA图谱可能至关重要。常用于物品采样的三种技术是:切割、擦拭和粘贴。粘贴采样技术具有一个优势,即它能够从犯罪现场物品上的选定区域(例如手套的内侧手指部位)采集潜在信息量很高的DNA来源——脱落的上皮细胞。此外,通过粘贴进行生物材料的表面采集减少了对已知PCR抑制剂(如衣物染料)的共采样。正确选择用于犯罪现场物品采样的胶带很重要。并非所有胶带都适合生物痕迹证据采集以及DNA提取。我们报告了一种符合这两个标准的胶带。文中呈现了三个不同案例,展示了对犯罪物品进行胶带采样的实用性。最后,讨论了胶带采集技术的优势,并给出了在各类案件物品上进行胶带采样的首选区域指南。