Nanomaterials & Solar Energy Conversion Lab, Department of Chemistry, National Institute of Technology, Tiruchirappalli 620 015, India.
J Pharm Biomed Anal. 2010 Nov 2;53(3):804-10. doi: 10.1016/j.jpba.2010.03.039. Epub 2010 Apr 3.
Spherical gold nanoparticles of approximately 16nm were synthesized using a sonochemical reduction method and characterized using UV-vis spectroscopy, atomic force microscopy (AFM) and transmission electron microscopy (TEM). The binding of these gold nanoparticles with bovine serum albumin (BSA) and human serum albumin (HSA) was investigated using UV-vis absorption and fluorescence spectroscopic techniques. A strong quenching of the fluorescence from serum albumins was observed due to the formation of a ground state complex with gold nanoparticles (static quenching). The fluorescence quenching constants, number of binding sites and binding constants were determined using Stern-Volmer and Benesi-Hildebrand plots. Using Forster Resonance Energy Transfer (FRET) theory, the distance between the donor (serum albumins) and acceptor (gold nanoparticles) was obtained, which showed that HSA has more affinity towards sonochemically synthesized gold nanoparticles compared to gold nanoparticles synthesized using other methods.
使用超声化学还原法合成了约 16nm 的球形金纳米粒子，并通过紫外-可见光谱、原子力显微镜（AFM）和透射电子显微镜（TEM）进行了表征。使用紫外-可见吸收和荧光光谱技术研究了这些金纳米粒子与牛血清白蛋白（BSA）和人血清白蛋白（HSA）的结合。由于形成了与金纳米粒子的基态配合物（静态猝灭），观察到血清白蛋白的荧光强烈猝灭。使用 Stern-Volmer 和 Benesi-Hildebrand 图确定了荧光猝灭常数、结合位点数和结合常数。使用Förster 共振能量转移（FRET）理论，获得了供体（血清白蛋白）和受体（金纳米粒子）之间的距离，结果表明与使用其他方法合成的金纳米粒子相比，HSA 对超声化学合成的金纳米粒子具有更高的亲和力。
