杂化金-单宁酸纳米颗粒与人血清白蛋白的相互作用。
Interactions of hybrid gold-tannic acid nanoparticles with human serum albumin.
作者信息
Sekowski Szymon, Tomaszewska Emilia, Soliwoda Katarzyna, Celichowski Grzegorz, Grobelny Jaroslaw
机构信息
Laboratory of Molecular Biophysics, Department of Biophysics, Institute of Biology, University of Bialystok, Ciolkowskiego 1 J, 15-245, Bialystok, Poland.
Department of Materials Technology and Chemistry, Faculty of Chemistry, University of Lodz, Pomorska 163, 90-236, Lodz, Poland.
出版信息
Eur Biophys J. 2017 Jan;46(1):49-57. doi: 10.1007/s00249-016-1134-1. Epub 2016 May 10.
Nanoparticles present a wide spectrum of chemical, biological, and physical properties which result in their usage in many branches of science. We present an investigation of the interaction between human serum albumin and hybrid gold-tannic acid nanoparticles synthesized via a chemical reduction method. The results obtained demonstrate that tannic acid can be a very effective reducing and stabilizing agent and allows monodisperse hybrid gold nanomaterial to be obtained. The synthesized hybrid gold-tannic acid nanoparticles strongly interact with human serum albumin by formation of protein-corona complexes. The strength of the interaction with albumin depends on the number of tannic acid molecules on the surface of the nanoparticles and the presence of citric acid. Nanoparticles of large size and rich in tannic acid react more strongly with the protein [K = (8.00 ± 0.2) × 10 M] compared with smaller ones [K = (6.83 ± 0.5) × 10 M] containing citric acid and low concentration of tannic acid.
纳米颗粒具有广泛的化学、生物和物理性质，这使得它们在许多科学领域都有应用。我们对通过化学还原法合成的金-单宁酸杂化纳米颗粒与人血清白蛋白之间的相互作用进行了研究。所得结果表明，单宁酸可以是一种非常有效的还原剂和稳定剂，能够制备出单分散的杂化金纳米材料。合成的金-单宁酸杂化纳米颗粒通过形成蛋白质冠层复合物与人血清白蛋白发生强烈相互作用。与白蛋白相互作用的强度取决于纳米颗粒表面单宁酸分子的数量以及柠檬酸的存在。与含有柠檬酸和低浓度单宁酸的较小纳米颗粒[K = (6.83 ± 0.5) × 10 M]相比，尺寸较大且富含单宁酸的纳米颗粒与蛋白质的反应更强[K = (8.00 ± 0.2) × 10 M]。
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