粪肠球菌 BMSZ711 产聚-N-乙酰葡糖胺：特性分析与生产优化。

Production of poly-N-acetylglucosamine by Staphylococcus saprophyticus BMSZ711: characterization and production optimization.

机构信息

Nano-Biomaterial Science Laboratory, Division of Applied Life Sciences (BK21), Graduate School and Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.

出版信息

Bioresour Technol. 2010 Sep;101(18):7188-91. doi: 10.1016/j.biortech.2010.03.141. Epub 2010 Apr 24.

DOI:10.1016/j.biortech.2010.03.141

PMID:20456948

Abstract

This is the first report on the characterization and production optimization of poly-N-acetylglucosamine (PNAG) in Staphylococcus saprophyticus. A strain producing glucosamine exopolysaccharide was isolated and characterized by biochemical test and 16S rRNA gene sequence homology analysis and named as S. saprophyticus BMSZ711. The molecular mass of the purified exopolymer was about 12 kDa. Digestion of the PNAG with DispersinB proved that it has beta-1,6 linkage. BMSZ711 can only produce PNAG when grown in M1 minimal medium but not in nutrient rich medium with optimum temperature of 30 degrees C and pH of 7. Glycerol and ammonium sulfate were found to be the best carbon and nitrogen source, respectively. Maximum PNAG production was obtained when glycerol 100mM, ammonium sulfate 0.3%, yeast extract 1.5 g/L, sodium chloride 10 g/L and valine 2mM were used.

摘要

这是关于葡萄球菌产聚-N-乙酰葡糖胺（PNAG）的特性和生产优化的首份报告。通过生化试验和 16S rRNA 基因序列同源性分析，我们分离并鉴定了一株产葡萄糖胺胞外多糖的菌株，并将其命名为腐生葡萄球菌 BMSZ711。纯化的胞外聚合物的分子量约为 12 kDa。用 DispersinB 消化 PNAG 证明其具有β-1,6 键。BMSZ711 只能在 M1 最小培养基中生长时产生 PNAG，而不能在营养丰富的培养基中生长，最佳温度为 30°C，pH 值为 7。甘油和硫酸铵分别是最佳的碳源和氮源。当使用 100mM 甘油、0.3%硫酸铵、1.5 g/L 酵母提取物、10 g/L 氯化钠和 2mM 缬氨酸时，PNAG 的产量最高。