Choi Jun Gu, Lee Youn Jeong, Kim Ji Yeon, Kim Yeon Hee, Paek Mi Ra, Yang Dong Kun, Son Seong Wan, Kim Jae Hong
National Veterinary Research and Quarantine Service, Anyang 430-757, Korea.
J Vet Sci. 2010 Jun;11(2):161-3. doi: 10.4142/jvs.2010.11.2.161.
In order to control the H9N2 subtype low pathogenic avian influenza (LPAI), an inactivated vaccine has been used in Korea since 2007. The Korean veterinary authority permitted the use of a single H9N2 LPAI vaccine strain to simplify the evolution of the circulating virus due to the immune pressure caused by the vaccine use. It is therefore important to determine the suitability of the vaccine strain in the final inactivated oil emulsion LPAI vaccine. In this study, we applied molecular rather than biological methods to verify the suitability of the vaccine strain used in commercial vaccines and successfully identified the strain by comparing the nucleotide sequences of the hemagglutinin and neuraminidase genes with that of the permitted Korean LPAI vaccine strain. It is thought that the method used in this study might be successfully applied to other viral genes of the LPAI vaccine strain and perhaps to other veterinary oil emulsion vaccines.
为了控制H9N2亚型低致病性禽流感（LPAI），韩国自2007年以来一直使用灭活疫苗。由于疫苗使用造成的免疫压力，韩国兽医当局允许使用单一的H9N2 LPAI疫苗株，以简化流行病毒的演变。因此，确定疫苗株在最终灭活油乳剂LPAI疫苗中的适用性很重要。在本研究中，我们应用分子方法而非生物学方法来验证商业疫苗中使用的疫苗株的适用性，并通过将血凝素和神经氨酸酶基因的核苷酸序列与韩国允许使用的LPAI疫苗株的序列进行比较，成功鉴定了该毒株。据认为，本研究中使用的方法可能成功应用于LPAI疫苗株的其他病毒基因，甚至可能应用于其他兽用油性乳剂疫苗。
