Villasana Laura, Pfankuch Timothy, Raber Jacob
Department of Behavioral Neuroscience, Oregon Health and Science University, Portland, OR 97239, USA.
Radiat Environ Biophys. 2010 Aug;49(3):421-6. doi: 10.1007/s00411-010-0290-4. Epub 2010 May 11.
Previously we found apoE isoform-dependent effects of (137)Cs irradiation on cognitive function of female mice 3 months following irradiation. Alterations in the number of immature neurons and in the levels of the dendritic marker microtubule-associated protein 2 (MAP-2) might contribute to the cognitive changes following irradiation. Therefore, in the present study we determined if, following (137)Cs irradiation, there are apoE isoform-dependent effects on loss of doublecortin-positive neuroprogenitor cells or MAP-2 immumonoreactivity. In the dentate gyrus, CA1 and CA3 regions of the hippocampus, enthorhinal and sensorimotor cortex, and central and basolateral nuclei of the amygdala of apoE3 female mice, MAP-2 immunoreactivity increased 3 months following (137)Cs irradiation. In addition, at 8 h following irradiation, the number of doublecortin-positive cells was higher in apoE3 than apoE2 or apoE4 mice. Together, these data indicate that brains of apoE3 mice respond differently to (137)Cs irradiation than those of apoE2 or apoE4 mice.
此前我们发现，¹³⁷Cs照射对雌性小鼠认知功能的影响存在载脂蛋白E（apoE）异构体依赖性，该影响在照射后3个月出现。未成熟神经元数量的改变以及树突标记物微管相关蛋白2（MAP-2）水平的改变可能导致照射后的认知变化。因此，在本研究中，我们确定¹³⁷Cs照射后，对双皮质素阳性神经祖细胞的损失或MAP-2免疫反应性是否存在apoE异构体依赖性影响。在apoE3雌性小鼠的海马齿状回、CA1和CA3区域、内嗅皮质和感觉运动皮质以及杏仁核的中央和基底外侧核中，¹³⁷Cs照射3个月后MAP-2免疫反应性增加。此外，照射后8小时，apoE3小鼠中双皮质素阳性细胞的数量高于apoE2或apoE4小鼠。这些数据共同表明，apoE3小鼠的大脑对¹³⁷Cs照射的反应与apoE2或apoE4小鼠不同。
