Bagnaninchi Pierre O
MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh - Scotland.
Int J Artif Organs. 2010 Apr;33(4):238-43.
To assess non-invasively and in real time the three- dimensional organization of cells within porous matrices by combining Fourier Domain Optical Coherence Tomography (FDOCT) and Impedance Spectroscopy (IS).
Broadband interferences resulting from the recombination of in-depth light scattering events within the sample and light from a reference arm are measured as a modulation of the spectrum generated by a superluminescent laser diode (lambdao = 930nm, FWHM 90nm). Fourier transform allows in-depth localization of the scatterers, and the 3D microstructure of the sample is reconstructed by raster scanning. Simultaneously impedance spectroscopy is performed with a dielectric probe connected to an impedance analyzer to gather additional cellular information, and synchronized with FDOCT measurements.
A combined IS-FDOCT system allowing an axial resolution of 5 micrometer in tissues and impedance measurements over the range 20MHz-1GHz has been developed. Alginate matrices have been characterized in terms of microstructure and impedance. Matrices seeded with adipose-derived stem cells have been monitored without the use of labeling agent.
We have developed a multimodality system that will be instrumental to non-invasively monitor changes in total cell volume fraction and infer cell-specific dielectric properties in 3D structure.
通过结合傅里叶域光学相干断层扫描（FDOCT）和阻抗谱（IS），对多孔基质内细胞的三维组织进行实时无创评估。
样品内深度光散射事件与参考臂光复合产生的宽带干涉，作为超发光激光二极管（λo = 930nm，半高宽90nm）产生的光谱调制进行测量。傅里叶变换可实现散射体的深度定位，并通过光栅扫描重建样品的三维微观结构。同时，使用连接到阻抗分析仪的介电探头进行阻抗谱测量，以收集额外的细胞信息，并与FDOCT测量同步。
已开发出一种组合式IS - FDOCT系统，其在组织中的轴向分辨率为5微米，可在20MHz - 1GHz范围内进行阻抗测量。已对藻酸盐基质的微观结构和阻抗进行了表征。在不使用标记剂的情况下，对接种了脂肪来源干细胞的基质进行了监测。
我们开发了一种多模态系统，该系统将有助于无创监测总细胞体积分数的变化，并推断三维结构中细胞特异性介电特性。
