Ma Hui-fang, Ren Xiu-jun, Tu Ya, Zhou Lei
Beijing University of Chinese Medicine, Beijing 100029, China.
Zhen Ci Yan Jiu. 2010 Feb;35(1):22-6.
To observe the effect of electroacupuncture (EA) on Calbindin D28 K(CB) expression and apoptosis in hippocampus in hyperlipemia rats with concomitant cerebral ischemia (CI).
Forty male SD rats were randomized into control, hyperlipemia+ CI (model), acupuncture I, and acupuncture II groups, with 10 cases in each. Hyperlipemia model was established by feeding the animals with high fat forage for 6 weeks and CI model established by occlusion of the unilateral middle cerebral artery. For rats of acupuncture I group, EA (1-3 mA, 15 Hz) was applied to bilateral "Sanyinjiao" (SP 6) and "Fenglong" (ST 40) for 20 min every time, once daily for 10 days before CI. For rats of both acupuncture I and II groups, after CI, EA was applied to SP 6, ST 40, "Baihui" (GV 20) and "Shuigou" (GV 26) were punctured with filiform needles and stimulated by twirled the needle with hand continuously for 1 min, once daily for 7 days. The apoptotic cells of hippocampal CA 3 area were displayed by TUNEL (terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling) method. CB antibody staining with immunohistochemistry was used to show CB-positive cells which were observed by optic microscope.
In comparison with control group, the percentage of apoptotic cells of the hippocampal CA 3 area in model group increased considerably (P < 0.01), while the number of CB immunoreaction (IR)-positive cells in the same area decreased significantly (P < 0.05). Compared with model group, the percentages of apoptotic cells of CA 3 area in acupuncture I and acupuncture II groups lowered remarkably (P < 0.01), while the numbers of CB IR-positive cells in the later two groups increased significantly (P < 0.01). The therapeutic effect of acupuncture I group was apparently superior to that of acupuncture II group in lowering the percentage of apoptotic cells and upregulating CB IR-positive cell number (P < 0.05).
Acupuncture can effectively lessen neuronal apoptosis of the hippocampus CA 3 area which may be closely related to its effect in upregulating the expression of CB in hyperlipemia + CI rats. Moreover, the cerebral ischemia injury degree may be lightened by acupuncture-lowering hyperlipemia first.
观察电针（EA）对高脂血症合并脑缺血（CI）大鼠海马中钙结合蛋白D28K（CB）表达及细胞凋亡的影响。
40只雄性SD大鼠随机分为对照组、高脂血症+CI（模型）组、针刺I组和针刺II组，每组10只。通过喂养高脂饲料6周建立高脂血症模型，采用结扎单侧大脑中动脉建立CI模型。针刺I组大鼠在CI前，每次将EA（1 - 3 mA，15 Hz）施加于双侧“三阴交”（SP 6）和“丰隆”（ST 40）20分钟，每天1次，共10天。针刺I组和针刺II组大鼠在CI后，将毫针针刺SP 6、ST 40，针刺“百会”（GV 20）和“水沟”（GV 26），并持续捻转手法刺激1分钟，每天1次，共7天。采用TUNEL（末端脱氧核苷酸转移酶介导的dUTP缺口末端标记）法显示海马CA 3区的凋亡细胞。用免疫组织化学法进行CB抗体染色以显示CB阳性细胞，在光学显微镜下观察。
与对照组相比，模型组海马CA 3区凋亡细胞百分比显著增加（P < 0.01），而同一区域CB免疫反应（IR）阳性细胞数量显著减少（P < 0.05）。与模型组相比，针刺I组和针刺II组CA 3区凋亡细胞百分比显著降低（P < 0.01），而后两组CB IR阳性细胞数量显著增加（P < 0.01）。在降低凋亡细胞百分比和上调CB IR阳性细胞数量方面，针刺I组的治疗效果明显优于针刺II组（P < 0.05）。
针刺可有效减轻海马CA 3区神经元凋亡，这可能与其上调高脂血症+CI大鼠CB表达的作用密切相关。此外，针刺先降低高脂血症可能减轻脑缺血损伤程度。
