Bu Yuan, Zhang Pei-ying, Geng De-qin, Cao Jin-xia, Li Ji-zhen
Department of Neurology, Xuzhou Hospital of Chinese Medicine, Xuzhou 221000, China.
Zhen Ci Yan Jiu. 2010 Jun;35(3):208-12.
To observe the effect of electroacupuncture (EA) plus oxygenmedicine (OM) on the expression of Bcl-2 and Bax in the hippocampal CA 1 area in cerebral ischemia/reperfusion injury (CI/RI) rats.
Thirty SD rats were randomized into sham-operation, model, EA, OM, EA+OM groups (n=6 /group). CI/RI model was established by using modified Pulsinelli 4 vessel occlusion and reperfusion. EA (100 Hz, 3.5 mA) was applied to "Baihui" (GV 20) and "Zusanli" (ST 36) 30 min, once daily for 4 days. Rats of OM and EA+ OM groups were put into a box filled with oxygen and atomized herbal medicines containing Bingpian (Borneolum), Shexiang (Moschus), Huangjing (Rhizoma Polygonati), Shouwu (Radix Polygoni Multiflori), etc. for 30 min, once daily for 4 days. Bcl-2 and Bax expression of the hippocampal CA 1 area was detected by immunohistochemistry.
Compared with sham group, the numbers of Bcl-2 immunoreaction (IR) and Bax IR positive cells, and the immunoactivity of Bcl-2 IR and Bax IR positive products in the hippocampal CA 1 area were increased significantly in model group (P < 0.05, P < 0.01). In comparison with model group, the number of Bcl-2 IR positive cells and Bcl-2 immunoactivity in EA, OM and EA+OM groups increased considerably (P < 0.01), while Bax IR positive cell numbers and Bax immunoactivity in EA, OM and OM+ EA groups decreased significantly (P < 0.01). The effects of EA+ OM were significantly superior to those of EA and OM groups in upregulating Bcl-2 IR positive cell number and Bcl-2 immunoactivity and downregulating Bax IR positive cell number and Bax immunoactivity (P < 0.01). No significant differences were found between EA and OM groups in the abovemen-EA and OM and EA+OM can effectively regulate the expression of Bcl-2 and Bax in tioned indexes (P > 0.05).
EA and OM and EA + OM can effectively regulate the expression of Bcl-2 and Bax in the hippocampal CA 1 area in CI/RI rats, and the effects of EA+OM are significantly superior to those of simple EA and simple OM, which may contribute to their effect in improving cerebral ischemia.
观察电针（EA）联合氧药（OM）对脑缺血/再灌注损伤（CI/RI）大鼠海马CA1区Bcl-2和Bax表达的影响。
将30只SD大鼠随机分为假手术组、模型组、电针组、氧药组、电针+氧药组（每组n = 6）。采用改良Pulsinelli四血管闭塞再灌注法建立CI/RI模型。电针（100Hz，3.5mA）针刺“百会”（GV 20）和“足三里”（ST 36）30分钟，每日1次，共4天。氧药组和电针+氧药组大鼠置于装有氧气和含冰片、麝香、黄精、何首乌等中药雾化剂的箱内30分钟，每日1次，共4天。采用免疫组化法检测海马CA1区Bcl-2和Bax的表达。
与假手术组比较，模型组海马CA1区Bcl-2免疫反应（IR）阳性细胞数、Bax IR阳性细胞数及Bcl-2 IR、Bax IR阳性产物免疫活性均显著增加（P < 0.05，P < 0.01）。与模型组比较，电针组、氧药组和电针+氧药组Bcl-2 IR阳性细胞数及Bcl-2免疫活性明显增加（P < 0.01），而电针组、氧药组和电针+氧药组Bax IR阳性细胞数及Bax免疫活性明显降低（P < 0.01）。电针+氧药组上调Bcl-2 IR阳性细胞数及Bcl-2免疫活性、下调Bax IR阳性细胞数及Bax免疫活性的作用明显优于电针组和氧药组（P < 0.01）。电针组和氧药组上述指标比较差异无统计学意义（P > 0.05）。
电针、氧药及电针+氧药均可有效调节CI/RI大鼠海马CA1区Bcl-2和Bax的表达，且电针+氧药组效果明显优于单纯电针组和单纯氧药组,这可能是其改善脑缺血作用的机制之一。
