Wang Zhu, Yang Yuexin, Xiang Xuesong, Zhu Yuming, Men Jianhua, He Mei
Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing.
Wei Sheng Yan Jiu. 2010 Mar;39(2):133-7, 142.
To evaluate the distribution and affecting factors of blood glucose in normal rats for the criterion of hyperglycemia in animal model.
The data of fast blood glucose (FBG) and 120 min postprandial blood glucose (PBG) of nearly 500 Wistar and SD rats from different experiment in our laboratory were collected and analyzed by multi-variable analysis and optimal scaling regression for the distribution and key affecting factors of blood glucose. Comparing the blood glucose determined by different method (glucose oxidase method or glucose monitor with strips), different duration (fasting for 5 h or 11 h), different blood source (from abdominal aorta or tail), and different time for separating serum after sampling (at once or till 120 min) was conducted in another 40-100 Wistar rats. A mathematical model for the contribution of different components was evaluated and combined by parameters of half width and range.
The average FBG and PBG of normal Wistar rats was (3.95 +/- 1.31) mmol/L and (5.65 +/- 1.63) mmol/L, and the 95% upper limit was 6.2 mmol/L and 7.9 mmol/L respectively. The differences of fasting duration, sample source, time before separating serum and testing methods, excepting for rat species, were significant (P < 0.05). The results showed that the blood glucose tested by glucose monitor method was not comparable with GOD method while the range of blood glucose was out of 7-11 mmol/L. The difference of blood glucose between fasting for 5h and 11 h was 0.8 mmol/L. Blood glucose from abdominal aorta was 40% higher than that from tail. The reduction of blood glucose was 8% in 60 min and over 50% in 120 min after blood being sampled. After the test requirement being regulated, the upper limit for FBG and PBG was expanded to 7.5 mmol/L and 10.4 mmol/L, respectively.
The onset of diabetics in rat is judged as blood glucose being higher than the expanded normal upper level. The criteria for rats are close to that for human.
评估正常大鼠血糖的分布及影响因素，为动物模型高血糖标准提供依据。
收集本实验室近500只不同实验的Wistar和SD大鼠的空腹血糖（FBG）及餐后120分钟血糖（PBG）数据，采用多变量分析和最优尺度回归分析血糖的分布及关键影响因素。另外选取40 - 100只Wistar大鼠，比较不同检测方法（葡萄糖氧化酶法或血糖仪试纸法）、不同禁食时长（禁食5小时或11小时）、不同采血部位（腹主动脉或尾静脉）以及采血后不同血清分离时间（立即分离或120分钟后分离）对血糖测定的影响。通过半峰宽和范围参数评估并组合不同成分的贡献数学模型。
正常Wistar大鼠的平均FBG和PBG分别为(3.95 ± 1.31) mmol/L和(5.65 ± 1.63) mmol/L，95%上限分别为6.2 mmol/L和7.9 mmol/L。除大鼠品系外，禁食时长、样本来源、血清分离前时间及检测方法的差异均具有统计学意义（P < 0.05）。结果显示，当血糖范围超出7 - 11 mmol/L时，血糖仪检测法与葡萄糖氧化酶法测定的血糖结果不可比。禁食5小时和11小时的血糖差值为0.8 mmol/L。腹主动脉采血的血糖比尾静脉采血高40%。采血后60分钟血糖降低8%，120分钟后降低超过50%。调整检测要求后，FBG和PBG的上限分别扩展至7.5 mmol/L和10.4 mmol/L。
大鼠糖尿病发病判定为血糖高于扩展后的正常上限水平。大鼠的标准与人的标准相近。
