Zhu Yuming, Ding Hong, Wang Zhu, Yang Yuexin, Xiang Xuesong
Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Wei Sheng Yan Jiu. 2010 Mar;39(2):168-71, 176.
To investigate the effect of epigallocatechin-3-gallate (EGCG) on the activity of alpha-glucosidase derived from bakers' yeast and small intestinal mucosa of Wistar rats, in order to establish an evaluation system for the activity of alpha-glucosidase inhibitor.
alpha-glucosidase was prepared from the bakers' yeast and the small intestinal mucosa of Wistar rats. The inhibitory activity of EGCG on alpha-glucosidase was based on the concentration of glucose in the reaction system.
EGCG was shown to be an alpha-glucosidase inhibitor. IC50 was 0.150 mg/ml and 0.837 mg/ml for alpha-glucosidase derived from bakers' yeast and small intestinal mucosa respectively.
The establishment of an in vitro evaluation system for AGI activity is conductive for screening alpha-glucosidase inhibitory substance. The AGI activity of EGCG is strong in vitro.
研究表没食子儿茶素没食子酸酯（EGCG）对面包酵母和Wistar大鼠小肠黏膜来源的α-葡萄糖苷酶活性的影响，以建立α-葡萄糖苷酶抑制剂活性评价体系。
从面包酵母和Wistar大鼠小肠黏膜中制备α-葡萄糖苷酶。EGCG对α-葡萄糖苷酶的抑制活性基于反应体系中葡萄糖的浓度。
EGCG被证明是一种α-葡萄糖苷酶抑制剂。面包酵母和小肠黏膜来源的α-葡萄糖苷酶的半数抑制浓度（IC50）分别为0.150mg/ml和0.837mg/ml。
建立AGI活性的体外评价体系有助于筛选α-葡萄糖苷酶抑制物质。EGCG在体外具有较强的AGI活性。
