Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA.
Nano Lett. 2010 Jun 9;10(6):2237-44. doi: 10.1021/nl1012147.
We demonstrate the feasibility of a nanopore based single-molecule DNA sequencing method, which employs multicolor readout. Target DNA is converted according to a binary code, which is recognized by molecular beacons with two types of fluorophores. Solid-state nanopores are then used to sequentially strip off the beacons, leading to a series of detectable photon bursts, at high speed. We show that signals from multiple nanopores can be detected simultaneously, allowing straightforward parallelization to large nanopore arrays.
我们展示了一种基于纳米孔的单分子 DNA 测序方法的可行性，该方法采用多色读出。目标 DNA 按照二进制代码进行转换，该代码由带有两种荧光团的分子信标识别。然后，固态纳米孔被用来依次去除信标，导致一系列可检测的光爆发，速度非常快。我们表明，可以同时检测多个纳米孔的信号，从而可以直接将其并行化到大型纳米孔阵列中。
