基于纳米孔的第四代DNA测序技术。

Nanopore-based fourth-generation DNA sequencing technology.

作者信息

Feng Yanxiao, Zhang Yuechuan, Ying Cuifeng, Wang Deqiang, Du Chunlei

机构信息

Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China; School of Physical Electronics, University of Electronic Science and Technology of China, Chengdu 611731, China.

出版信息

Genomics Proteomics Bioinformatics. 2015 Feb;13(1):4-16. doi: 10.1016/j.gpb.2015.01.009. Epub 2015 Mar 2.

DOI:10.1016/j.gpb.2015.01.009

PMID:25743089

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4411503/

Abstract

Nanopore-based sequencers, as the fourth-generation DNA sequencing technology, have the potential to quickly and reliably sequence the entire human genome for less than $1000, and possibly for even less than $100. The single-molecule techniques used by this technology allow us to further study the interaction between DNA and protein, as well as between protein and protein. Nanopore analysis opens a new door to molecular biology investigation at the single-molecule scale. In this article, we have reviewed academic achievements in nanopore technology from the past as well as the latest advances, including both biological and solid-state nanopores, and discussed their recent and potential applications.

摘要

基于纳米孔的测序仪作为第四代DNA测序技术，有潜力以低于1000美元的成本，甚至可能低于100美元的成本快速且可靠地对整个人类基因组进行测序。该技术所采用的单分子技术使我们能够进一步研究DNA与蛋白质之间以及蛋白质与蛋白质之间的相互作用。纳米孔分析为单分子尺度的分子生物学研究打开了一扇新的大门。在本文中，我们回顾了过去纳米孔技术的学术成果以及最新进展，包括生物纳米孔和固态纳米孔，并讨论了它们近期和潜在的应用。