A U-tetrad 稳定人端粒 RNA G-四链体结构。

A U-tetrad stabilizes human telomeric RNA G-quadruplex structure.

机构信息

Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.

出版信息

J Am Chem Soc. 2010 Jun 2;132(21):7231-3. doi: 10.1021/ja909708a.

DOI:10.1021/ja909708a

PMID:20459096

Abstract

Telomeric repeat-containing RNA is a new noncoding RNA molecule recently discovered in mammalian cells. Here we report the structural features of human telomere RNA r(UAGGGU) in the presence of K(+) and Na(+). We demonstrated for the first time that a novel U-tetrad is formed at the 3' end of a parallel human telomeric RNA G-quadruplex. The U-tetrad dramatically stabilizes human telomeric RNA G-quadruplex structure, leading to an increase in melting temperature (T(m)) of 29 degrees C. The U-tetrad-stabilized telomeric RNA G-quadruplex structure adds considerably to our understanding of the diversity of RNA G-quadruplex architectures. It shows that the structure of base "quartets" is important in RNA assembly. The structural information will be invaluable for understanding the function of human telomere RNA.

摘要

端粒重复 RNA 是一种最近在哺乳动物细胞中发现的新型非编码 RNA 分子。在这里，我们报告了在存在 K(+)和 Na(+)的情况下人端粒 RNA r(UAGGGU)的结构特征。我们首次证明，在平行的人端粒 RNA G-四链体的 3' 末端形成了一个新的 U-四联体。U-四联体显著稳定了人端粒 RNA G-四链体结构，导致熔点 (T(m))升高 29 摄氏度。U-四联体稳定的端粒 RNA G-四链体结构极大地增加了我们对 RNA G-四链体结构多样性的理解。它表明碱基“四联体”的结构在 RNA 组装中很重要。该结构信息对于理解人端粒 RNA 的功能将是非常宝贵的。