Chalmers University of Technology, Department of Chemical and Biological Engineering, Kemivagen 10, 412 96 Goteborg, Sweden.
J Biomed Opt. 2010 Mar-Apr;15(2):026026. doi: 10.1117/1.3374039.
A compact high-power fiber-based femtosecond laser system is presented for coherent anti-Stokes Raman scattering/second-harmonic generation (CARS/SHG) microscopy, and quantitatively compared with a conventional picosecond optical parametric oscillator (OPO)-based system. While the broad spectral width of the femtosecond pulses results in 2.5 times lower image contrast and limited spectral selectivity, lipid stores, myosin, and collagen filaments in living cells can clearly be identified at 60 times lower excitation powers compared to the picosecond system. Visually the images contain the same information. Together with simple operation, small footprint, and low cost, the capabilities of this high-power all-fiber-based laser system promise a more general use of nonlinear microscopy within the biosciences.
本文介绍了一种紧凑的高功率纤维飞秒激光系统，用于相干反斯托克斯拉曼散射/二次谐波产生（CARS/SHG）显微镜，并与传统的皮秒光参量振荡器（OPO）基系统进行了定量比较。虽然飞秒脉冲的光谱宽度较宽，但图像对比度降低了 2.5 倍，光谱选择性有限，但与皮秒系统相比，活细胞中的脂质储存、肌球蛋白和胶原蛋白丝可以在低 60 倍的激发功率下清晰识别。从视觉上看，这些图像包含相同的信息。该高功率全光纤激光系统具有操作简单、占地面积小、成本低等特点，有望在生物科学中更广泛地应用非线性显微镜。
