Campbell W F, Kufe D W, Peters W P, Spiegelman S, Frankel J W
Intervirology. 1978;10(1):11-23. doi: 10.1159/000148963.
Marek's disease herpesvirus (MDHV) isolated from chickens free of naturally occurring avian leukosis virus (ALV) infection produced characteristic foci in both chicken embryo fibroblast (CEF) and chicken kidney cell (CKC) cultures. MDHV-A, which was extracted from the feather follicle epithelium of chickens naturally infected with ALV, did not induce cytologic changes in CEF cultures, but did cause focus formation in CK cultures. ALV was detected in MDHV-A, but not in MDHV preparations. MDHV-A (reconstructed in vivo) and MDHV were further distinguished from one another by inoculation of ALV-free LSI-SPF chickens. MDHV-A elicited a high incidence of early mortality which was not accompanied by the gross tumor spectrum characteristic of Marek's disease, although extensive histologic lesions were present. The differences between MDHV and MDHV-A were not as striking in another line of ALV-free chickens (SPAFAS). By contrast, among conventional chickens with naturally occurring ALV infection, neither MDHV-A nor MDHV caused appreciable early mortality although both were highly oncogenic (gross tumor development). These observations demonstrate that the presence of an oncornavirus (ALV), detected by radioimmune but not complement fixation assays, can influence the in vitro and in vivo characteristics of an oncogenic herpesvirus (MDHV). The observations recorded here resolve some of the inconsistencies reported in the literature. Thus, the apparent failure by some to find interactions between MDHV and oncornaviruses can be ascribed to the comparatively limited sensitivity of the complement fixation assay used to detect oncornaviruses (ALV). We have shown that the presence of oncornavirus detectable by radioimmune assay, but not by complement fixation, can influence the in vitro and in vivo responses of an oncogenic herpesvirus (MDHV). Our observations relating to viral interaction do not imply that MDHV required the presence of ALV to produce disease.
从无自然感染禽白血病病毒（ALV）的鸡中分离出的马立克氏病疱疹病毒（MDHV），在鸡胚成纤维细胞（CEF）和鸡肾细胞（CKC）培养物中均产生特征性病灶。从自然感染ALV的鸡的毛囊上皮中提取的MDHV-A，在CEF培养物中未诱导细胞学变化，但在CK培养物中确实引起病灶形成。在MDHV-A中检测到了ALV，但在MDHV制剂中未检测到。通过接种无ALV的LSI-SPF鸡，进一步区分了MDHV-A（体内重建）和MDHV。MDHV-A引发了高早期死亡率，尽管存在广泛的组织学病变，但并未伴随马立克氏病特有的大体肿瘤谱。在另一品系的无ALV鸡（SPAFAS）中，MDHV和MDHV-A之间的差异并不那么明显。相比之下，在自然感染ALV的常规鸡中，MDHV-A和MDHV均未引起明显的早期死亡，尽管两者都具有高度致癌性（大体肿瘤发展）。这些观察结果表明，通过放射免疫而非补体结合试验检测到的肿瘤病毒（ALV）的存在，可影响致癌疱疹病毒（MDHV）的体外和体内特征。此处记录的观察结果解决了文献中报道的一些不一致之处。因此，一些人未能发现MDHV与肿瘤病毒之间相互作用的明显原因，可归因于用于检测肿瘤病毒（ALV）的补体结合试验相对有限的敏感性。我们已经表明，通过放射免疫试验可检测到但补体结合试验检测不到的肿瘤病毒的存在，可影响致癌疱疹病毒（MDHV）的体外和体内反应。我们关于病毒相互作用的观察结果并不意味着MDHV需要ALV的存在才能引发疾病。
