Maie S, Nakamura K, Ichihara K, Abiko Y
Department of Pharmacology, Asahikawa Medical College, Japan.
Jpn Circ J. 1991 Apr;55(4):336-42. doi: 10.1253/jcj.55.336.
The effect of human superoxide dismutase (h-SOD) on the ischemic heart was studied in the isolated perfused working rat heart. Myocardial mechanical function expressed as pressure-rate product decreased and completely stopped within 5 min after the onset of global ischemia, and never recovered after reperfusion following 20 min of ischemia. In the ischemic myocardium, the levels of ATP, ADP, and creatine phosphate decreased, and those of AMP and lactate increased. Reperfusion of the ischemic heart did not restore the level of ATP completely. When the heart was treated with h-SOD, the perfusion medium was switched from the buffer containing no h-SOD to that containing h-SOD at either 100, 300 or 1,000 units/ml 5 min before the onset of ischemia. The pressure-rate products of the heart treated with 100, 300, and 1,000 units/ml of h-SOD were restored by reperfusion to 22%, 59%, and 51% of the preischemic level, respectively. The levels of ATP and creatine phosphate in the reperfused heart with 300 and 1,000 units/ml of h-SOD were significantly higher than those without h-SOD. However, a dose-response relationship was not observed when h-SOD was used in concentrations greater than 300 units/ml. These results indicate that a certain amount of h-SOD has some beneficial effects on the ischemic myocardium.
在离体灌注的工作大鼠心脏中研究了人超氧化物歧化酶（h-SOD）对缺血心脏的影响。以压力-心率乘积表示的心肌机械功能在全心缺血开始后5分钟内下降并完全停止，缺血20分钟后再灌注，心肌机械功能未恢复。在缺血心肌中，ATP、ADP和磷酸肌酸水平降低，而AMP和乳酸水平升高。缺血心脏再灌注后，ATP水平未完全恢复。当心脏用h-SOD处理时，在缺血开始前5分钟将灌注培养基从不含h-SOD的缓冲液换成含100、300或1000单位/毫升h-SOD的缓冲液。用100、300和1000单位/毫升h-SOD处理的心脏，再灌注后压力-心率乘积分别恢复到缺血前水平的22%、59%和51%。300和1000单位/毫升h-SOD处理的再灌注心脏中ATP和磷酸肌酸水平显著高于未用h-SOD处理的心脏。然而，当h-SOD浓度大于300单位/毫升时，未观察到剂量-反应关系。这些结果表明，一定量的h-SOD对缺血心肌有一些有益作用。
