Ye Fei, Song Cun-Jiang, Tao Jian, Li Chang-Lin
Ying Yong Sheng Tai Xue Bao. 2010 Feb;21(2):386-90.
By the method of Biolog, a comparative study was made on the utilization level of single carbon source by the microbes in the rhizosphere soils of two transgenic cottons and their parents, aimed to approach the effects of planting transgenic cotton on the functional diversity of rhizosphere soil microbial community. Compared with planting non-transgenic cotton, planting transgenic cotton had less effects on the carbon sourceing utilization ability, Shannon functional diversity index, and evenness index of rhizosphere soil microbes at seedling, squaring, boll-opening, and senescence stages, but decreased the carbon source utilization ability and functional diversity index at flowering and boll-forming stage significantly. Principal component analysis (PCA) indicated that there existed greater differentiation in the carbon source utilization by the microbes in rhizosphere soils of nontransgenic and transgenic cottons at flowering and boll-forming stage, suggesting the significant difference in the carbon source utilization pattern of the microbes at this stage.
采用Biolog方法，对两种转基因棉花及其亲本根际土壤中微生物对单一碳源的利用水平进行了比较研究，旨在探讨种植转基因棉花对根际土壤微生物群落功能多样性的影响。与种植非转基因棉花相比，种植转基因棉花对苗期、蕾期、开花期和衰老期根际土壤微生物的碳源利用能力、香农功能多样性指数和均匀度指数影响较小，但在开花结铃期显著降低了碳源利用能力和功能多样性指数。主成分分析（PCA）表明，非转基因和转基因棉花根际土壤微生物在开花结铃期对碳源的利用存在较大差异，表明该阶段微生物碳源利用模式存在显著差异。
