基于放射性核素成像验证的活体小鼠生物发光断层成像。
In vivo mouse bioluminescence tomography with radionuclide-based imaging validation.
机构信息
Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
出版信息
Mol Imaging Biol. 2011 Feb;13(1):53-8. doi: 10.1007/s11307-010-0332-y.
INTRODUCTION
Bioluminescence imaging, especially planar bioluminescence imaging, has been extensively applied in in vivo preclinical biological research. Bioluminescence tomography (BLT) has the potential to provide more accurate imaging information due to its 3D reconstruction compared with its planar counterpart.
METHODS
In this work, we introduce a positron emission tomography (PET) radionuclide imaging-based strategy to validate the BLT results. X-ray computed tomography, PET, spectrally resolved bioluminescence imaging, and surgical excision were performed on a tumor xenograft mouse model expressing a bioluminescent reporter gene.
RESULTS
With different spectrally resolved measured data, the BLT reconstructions were acquired based on the third-order simplified spherical harmonics (SP3) approximation and the diffusion approximation (DA). The corresponding tomographic images were obtained for validation of bioluminescence source reconstruction.
CONCLUSION
Our results show the strength of PET imaging compared with other validation methods for BLT and improved source localization accuracy based on the SP(3) approximation compared with the diffusion approximation.
简介
生物发光成像是一种在体临床医学前生物研究中广泛应用的技术，特别是平面生物发光成像。与平面成像相比，生物发光断层成像（BLT）具有 3D 重建的优势，因此有可能提供更准确的成像信息。
方法
在这项工作中，我们引入了一种基于正电子发射断层扫描（PET）放射性核素成像的策略来验证 BLT 结果。在表达生物发光报告基因的肿瘤异种移植小鼠模型上进行了 X 射线计算机断层扫描、PET、光谱分辨生物发光成像和手术切除。
结果
利用不同的光谱分辨测量数据，基于三阶简化球谐函数（SP3）逼近和扩散逼近（DA）获取 BLT 重建。获得相应的断层图像以验证生物发光源重建。
结论
与其他 BLT 验证方法相比，我们的结果显示了 PET 成像的优势，并基于 SP(3)逼近与扩散逼近相比，提高了源定位精度。
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