Dehghani Hamid, Davis Scott C, Jiang Shudong, Pogue Brian W, Paulsen Keith D, Patterson Michael S
Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA.
Opt Lett. 2006 Feb 1;31(3):365-7. doi: 10.1364/ol.31.000365.
Spectrally resolved bioluminescence optical tomography is an approach to recover images of luciferase activity within a volume using multiwavelength emission data from internal bioluminescence sources. The underlying problem of uniqueness associated with nonspectrally resolved intensity-based bioluminescence tomography is highlighted. Reconstructed images of bioluminescence are presented by using as input both simulated and real multiwavelength data from a tissue-simulating phantom. The location of the internal bioluminescence is obtained with 1 mm accuracy. Further, the amplitude of the reconstructed source is proportional to the actual bioluminescence intensity.
光谱分辨生物发光光学断层扫描是一种利用来自内部生物发光源的多波长发射数据来恢复体积内荧光素酶活性图像的方法。突出了与非光谱分辨基于强度的生物发光断层扫描相关的唯一性这一潜在问题。通过使用来自组织模拟体模的模拟和真实多波长数据作为输入，呈现了生物发光的重建图像。内部生物发光的位置以1毫米的精度获得。此外，重建源的幅度与实际生物发光强度成正比。
