Pediatric Research Institute, University Hospital, Rikshospitalet, Oslo, Norway.
Scand J Clin Lab Invest. 1972;29(4):453-9. doi: 10.3109/00365517209080265.
The effect of pH on bilirubin binding by human erythrocytes was studied by incubating erythrocytes with solutions of bilirubin and albumin at pH levels 7.4-6.8. A reduction in pH from 7.4 greatly increased the cellular binding of bilirubin at all molar bilirubin/albumin ratios, even below the 1/1 limit. Spectrophotometric studies indicated that mainly the spectrum of the bilirubin molecule bound at the second binding site on the albumin molecule is affected by low pH. When cells were incubated with bilirubin without albumin, increased cellular binding of bilirubin at low pH was also found. However, the bilirubin concentrations greatly exceeded the solubility of bilirubin. The results and their possible clinical implications are discussed.
研究了 pH 值对人红细胞胆红素结合的影响，方法是将胆红素和白蛋白溶液在 pH 值为 7.4-6.8 条件下孵育。从 pH 值 7.4 降低到 6.8 大大增加了所有摩尔比胆红素/白蛋白的细胞结合胆红素，甚至低于 1/1 限制。分光光度研究表明，主要是结合在白蛋白分子上的第二个结合部位的胆红素分子的光谱受到低 pH 值的影响。当细胞在没有白蛋白的情况下与胆红素孵育时，也发现低 pH 值时细胞结合胆红素增加。然而，胆红素浓度大大超过了胆红素的溶解度。讨论了这些结果及其可能的临床意义。
