Pediatric Research Institute, University Hospital, Rikshospitalet, Oslo, Norway.
Scand J Clin Lab Invest. 1972;29(4):461-5. doi: 10.3109/00365517209080266.
A clonal strain of rat hepatoma cells (MH₁C₁) known to take up and conjugate bilirubin was incubated in media containing bilirubin and albumin in different molar ratios at a constant bilirubin concentration. The highest rate of bilirubin conjugation was found at a molar ratio of 1/1. Excess bilirubin markedly reduced both the conjugation of bilirubin and the incorporation of [14C]alanine, indicating a toxic effect on the cells. Excess albumin also depressed the formation of bilirubin conjugates, but incorporation of [14C]alanine proceeded at a normal rate, indicating that the reduced conjugation is probably due to stronger binding of bilirubin to albumin and reduced cellular uptake of bilirubin. Bilirubin bound to albumin at a molar ratio of 1/1 had no toxic effects on the cells as judged by cell morphology and conjugation capacity, even at a total bilirubin concentration as high as 680 μmol/l.
已知能摄取和结合胆红素的大鼠肝癌细胞（MH₁C₁）克隆株在含有不同摩尔比胆红素和白蛋白的培养基中，于恒定胆红素浓度下孵育。在摩尔比为 1/1 时，胆红素结合的速度最快。多余的胆红素明显降低了胆红素的结合和[14C]丙氨酸的掺入，表明对细胞有毒性作用。多余的白蛋白也抑制了胆红素结合物的形成，但[14C]丙氨酸的掺入仍以正常速度进行，表明减少的结合可能是由于胆红素与白蛋白的结合更强，以及胆红素向细胞内摄取减少。从细胞形态和结合能力判断，摩尔比为 1/1 结合的胆红素对细胞没有毒性作用，即使总胆红素浓度高达 680 μmol/l 也是如此。
