肝螺杆菌 Hh0072 基因编码一种新型的 CAZy GT11 家族的 alpha1-3-岩藻糖基转移酶。
Helicobacter hepaticus Hh0072 gene encodes a novel alpha1-3-fucosyltransferase belonging to CAZy GT11 family.
机构信息
Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA.
出版信息
Glycobiology. 2010 Sep;20(9):1077-88. doi: 10.1093/glycob/cwq068. Epub 2010 May 6.
Abstract
Lewis x (Le(x)) and sialyl Lewis x (SLe(x))-containing glycans play important roles in numerous physiological and pathological processes. The key enzyme for the final step formation of these Lewis antigens is alpha1-3-fucosyltransferase. Here we report molecular cloning and functional expression of a novel Helicobacter hepaticus alpha1-3-fucosyltransferase (HhFT1) which shows activity towards both non-sialylated and sialylated Type II oligosaccharide acceptor substrates. It is a promising catalyst for enzymatic and chemoenzymatic synthesis of Le(x), sialyl Le(x) and their derivatives. Unlike all other alpha1-3/4-fucosyltransferases characterized so far which belong to Carbohydrate Active Enzyme (CAZy, http://www.cazy.org/) glycosyltransferase family GT10, the HhFT1 shares protein sequence homology with alpha1-2-fucosyltransferases and belongs to CAZy glycosyltransferase family GT11. The HhFT1 is thus the first alpha1-3-fucosyltransferase identified in the GT11 family.
摘要
Lewis x (Le(x)) 和唾液酸化 Lewis x (SLe(x)) 含有聚糖在许多生理和病理过程中发挥重要作用。这些 Lewis 抗原的最后一步形成的关键酶是α1-3-岩藻糖基转移酶。在这里，我们报道了一种新型的嗜胆菌α1-3-岩藻糖基转移酶（HhFT1）的分子克隆和功能表达，它对非唾液酸化和唾液酸化的 II 型寡糖受体底物均具有活性。它是酶法和化学酶法合成 Le(x)、唾液酸化 Le(x)及其衍生物的有前途的催化剂。与迄今为止所有属于碳水化合物活性酶（CAZy，http://www.cazy.org/）糖基转移酶家族 GT10 的其他α1-3/4-岩藻糖基转移酶不同，HhFT1 与α1-2-岩藻糖基转移酶具有蛋白质序列同源性，属于 CAZy 糖基转移酶家族 GT11。因此，HhFT1 是 GT11 家族中鉴定的第一个α1-3-岩藻糖基转移酶。
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本文引用的文献
1
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J Am Chem Soc. 2009 Dec 30;131(51):18467-77. doi: 10.1021/ja907750r.
2
Chemoenzymatic synthesis of GDP-L-fucose and the Lewis X glycan derivatives.GDP-L-岩藻糖及Lewis X聚糖衍生物的化学酶法合成
Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16096-101. doi: 10.1073/pnas.0908248106. Epub 2009 Sep 4.
3
Remodeling bacterial polysaccharides by metabolic pathway engineering.通过代谢途径工程改造细菌多糖
Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4207-12. doi: 10.1073/pnas.0812432106. Epub 2009 Feb 26.
4
The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics.碳水化合物活性酶数据库（CAZy）：糖原组学的专业资源。
Nucleic Acids Res. 2009 Jan;37(Database issue):D233-8. doi: 10.1093/nar/gkn663. Epub 2008 Oct 5.
5
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6
Combinatorial chemoenzymatic synthesis and high-throughput screening of sialosides.唾液酸苷的组合化学酶法合成与高通量筛选
ACS Chem Biol. 2008 Sep 19;3(9):567-76. doi: 10.1021/cb800127n. Epub 2008 Aug 27.
7
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8
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