Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA.
Glycobiology. 2010 Sep;20(9):1077-88. doi: 10.1093/glycob/cwq068. Epub 2010 May 6.
Lewis x (Le(x)) and sialyl Lewis x (SLe(x))-containing glycans play important roles in numerous physiological and pathological processes. The key enzyme for the final step formation of these Lewis antigens is alpha1-3-fucosyltransferase. Here we report molecular cloning and functional expression of a novel Helicobacter hepaticus alpha1-3-fucosyltransferase (HhFT1) which shows activity towards both non-sialylated and sialylated Type II oligosaccharide acceptor substrates. It is a promising catalyst for enzymatic and chemoenzymatic synthesis of Le(x), sialyl Le(x) and their derivatives. Unlike all other alpha1-3/4-fucosyltransferases characterized so far which belong to Carbohydrate Active Enzyme (CAZy, http://www.cazy.org/) glycosyltransferase family GT10, the HhFT1 shares protein sequence homology with alpha1-2-fucosyltransferases and belongs to CAZy glycosyltransferase family GT11. The HhFT1 is thus the first alpha1-3-fucosyltransferase identified in the GT11 family.
Lewis x (Le(x)) 和唾液酸化 Lewis x (SLe(x)) 含有聚糖在许多生理和病理过程中发挥重要作用。这些 Lewis 抗原的最后一步形成的关键酶是α1-3-岩藻糖基转移酶。在这里，我们报道了一种新型的嗜胆菌α1-3-岩藻糖基转移酶（HhFT1）的分子克隆和功能表达，它对非唾液酸化和唾液酸化的 II 型寡糖受体底物均具有活性。它是酶法和化学酶法合成 Le(x)、唾液酸化 Le(x)及其衍生物的有前途的催化剂。与迄今为止所有属于碳水化合物活性酶（CAZy，http://www.cazy.org/）糖基转移酶家族 GT10 的其他α1-3/4-岩藻糖基转移酶不同，HhFT1 与α1-2-岩藻糖基转移酶具有蛋白质序列同源性，属于 CAZy 糖基转移酶家族 GT11。因此，HhFT1 是 GT11 家族中鉴定的第一个α1-3-岩藻糖基转移酶。
