Jenkins M C, Parker C, Hill D, Pinckney R D, Dyer R, Dubey J P
United States Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, Building 1040, BARC-EAST, Beltsville, MD 20705, USA.
Vet Parasitol. 2007 Jan 31;143(2):161-5. doi: 10.1016/j.vetpar.2006.08.011. Epub 2006 Sep 25.
The role of rodents in the epidemiology of neosporosis was investigated by assaying brain tissue of feral mice (Mus musculus) and rats (Rattus norvegicus) for Neospora caninum. Both mouse and rat brain tissue were extracted for total DNA, and subjected to two different N. caninum-specific nested polymerase chain reaction (PCR) assays. A portion of brain tissue from the mice and rats were also assayed for N. caninum in gerbils or gamma-interferon gene knockout (KO) mice. Of the 105 feral mice tested, 10% were positive in the N. caninum-specific PCR assays. Of the 242 rats tested, 30% were positive in both assays. Although mice and rats had N. caninum by PCR testing, clinical signs of N. caninum infection were not observed nor were N. caninum parasites observed in gerbils or KO mice inoculated with the rodent brain tissue.
通过检测野生小鼠（小家鼠）和大鼠（褐家鼠）脑组织中的犬新孢子虫，研究了啮齿动物在新孢子虫病流行病学中的作用。从小鼠和大鼠脑组织中提取总DNA，并进行两种不同的犬新孢子虫特异性巢式聚合酶链反应（PCR）检测。还对小鼠和大鼠的部分脑组织在沙鼠或γ干扰素基因敲除（KO）小鼠中进行犬新孢子虫检测。在检测的105只野生小鼠中，10%在犬新孢子虫特异性PCR检测中呈阳性。在检测的242只大鼠中，30%在两种检测中均呈阳性。虽然通过PCR检测小鼠和大鼠感染了犬新孢子虫，但未观察到犬新孢子虫感染的临床症状，在用啮齿动物脑组织接种的沙鼠或KO小鼠中也未观察到犬新孢子虫寄生虫。
