The Department of Chemistry, Loyola University-Chicago, Chicago, IL 60626, USA.
Nanotechnology. 2010 Jun 11;21(23):235105. doi: 10.1088/0957-4484/21/23/235105. Epub 2010 May 17.
A strategy to bind bacterial cells to surfaces in a directed fashion via dip-pen nanolithography (DPN) is presented. Cellular attachment to pre-designed DPN generated microarrays was found to be dependent on the shape and size of the surface feature. While this observation is likely due in part to a dense, well formed mercaptohexadecanoic acid (MHA) monolayer generated via DPN, it may also simply be due to the physical shape of the surface structure. Motile Pseudomonas aeruginosa bacterial cells were observed to bind to DPN generated mercaptohexadecanoic acid/poly-L-lysine (MHA/PLL) line patterns, 'blocks' made up of eight lines with 100 nm spacings, with approximately 80% occupancy. Cellular binding to these 'block' surface structures occurs via an electrostatic interaction between negatively charged groups on the bacterial cell surface and positively charged poly-L-lysine (PLL) assemblies. These data indicate that these DPN generated 'block' surface structures provide a promising footprint for the attachment of motile bacterial cells that may find utility in cell based biosensors or single cell studies.
本文提出了一种通过蘸笔纳米光刻（DPN）将细菌细胞定向结合到表面的策略。发现细胞附着到预先设计的 DPN 生成的微阵列上取决于表面特征的形状和大小。虽然这种观察结果可能部分归因于通过 DPN 生成的密集、良好形成的巯基十六烷酸（MHA）单层，但也可能仅仅是由于表面结构的物理形状。观察到运动性铜绿假单胞菌细菌细胞结合到 DPN 生成的巯基十六烷酸/聚-L-赖氨酸（MHA/PLL）线图案，“块”由 8 条线组成，间距为 100nm，占有率约为 80%。细胞与这些“块”表面结构的结合是通过细菌细胞表面带负电荷的基团与带正电荷的聚-L-赖氨酸（PLL）组装体之间的静电相互作用发生的。这些数据表明，这些 DPN 生成的“块”表面结构为附着运动性细菌细胞提供了有前途的基础，这些细胞可能在基于细胞的生物传感器或单细胞研究中找到用途。
