Aksamit R R, Long C W
J Gen Virol. 1978 Mar;38(3):419-29. doi: 10.1099/0022-1317-38-3-419.
Cones of thioguanine resistant K-BALB mouse cells wereisolated which were inducible for endogenous type C virus synthesis by cycloheximide and dexamethsone, but not 5-iododeoxyuridine. A comparison of the number of foci formed on NRK and SC-I cells suggested that the xenotropic virus was suppressed. The variants were not defective in the incorporation of thymidine or iododeoxyuridine or deficient in thymidine kinase, but were deficient in hypoxanthine-guanine phosphoribosyltransferase and the incorporation of hypoxanthine into nucleic acid. Because these cells are blocked at some point in the expression of endogenous virus, they may prove useful in establishing the steps involved in chemical activation of virus synthesis.
分离出对硫鸟嘌呤耐药的K-BALB小鼠细胞克隆，这些克隆可被环己酰亚胺和地塞米松诱导内源性C型病毒合成，但不能被5-碘脱氧尿苷诱导。比较在NRK和SC-I细胞上形成的病灶数量表明，嗜异性病毒受到抑制。这些变体在胸苷或碘脱氧尿苷掺入方面没有缺陷，也不缺乏胸苷激酶，但缺乏次黄嘌呤-鸟嘌呤磷酸核糖转移酶，且次黄嘌呤掺入核酸存在缺陷。由于这些细胞在内源性病毒表达的某个点上被阻断，它们可能有助于确定病毒合成化学激活所涉及的步骤。
