Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
Korean J Physiol Pharmacol. 2010 Apr;14(2):71-5. doi: 10.4196/kjpp.2010.14.2.71. Epub 2010 Apr 30.
To investigate the intestinal absorption of a fibrinolytic and proteolytic lumbrokinase extracted from Eisenia andrei, we used rat everted gut sacs and an in situ closed-loop recirculation method. We extracted lumbrokinase from Eisenia andrei, and then raised polyclonal antibody against lumbrokinase. Fibrinolytic activity and proteolytic activity in the serosal side of rat everted gut sacs incubated with lumbrokinase showed dose- and time-dependent patterns. Immunological results obtained by western blotting serosal side solution using rat everted gut sacs method showed that lumbrokinase proteins between 33.6 and 54.7 kDa are absorbed mostly by the intestinal epithelium. Furthermore, MALDI-TOF mass spectrometric analysis of plasma fractions obtained by in situ recirculation method confirmed that lumbrokinase F1 is absorbed into blood. These results support the notion that lumbrokinase can be absorbed from mucosal lumen into blood by oral administration.
为了研究从赤子爱胜蚓中提取的纤溶酶和蛋白水解酶蚓激酶的肠道吸收情况，我们使用大鼠外翻肠囊和原位闭路循环法进行了研究。我们从赤子爱胜蚓中提取了蚓激酶，然后制备了针对蚓激酶的多克隆抗体。在含有蚓激酶的大鼠外翻肠囊孵育物的浆膜侧，纤溶活性和蛋白水解活性呈现出剂量和时间依赖性模式。使用大鼠外翻肠囊法通过 Western 印迹法对浆膜侧溶液进行的免疫学结果表明，33.6 至 54.7 kDa 之间的蚓激酶蛋白主要被肠上皮吸收。此外，原位再循环法获得的血浆级分的 MALDI-TOF 质谱分析证实，蚓激酶 F1 被吸收到血液中。这些结果支持蚓激酶可以通过口服从黏膜腔吸收到血液中的观点。
