Dunnington E A, Gal O, Siegel P B, Haberfeld A, Cahaner A, Lavi U, Plotsky Y, Hillel J
Poultry Science Department, Virginia Polytechnic Institute, Blacksburg 24061-0332.
Poult Sci. 1991 Mar;70(3):463-7. doi: 10.3382/ps.0700463.
A pair of lines of White Plymouth Rock chickens selected for high or low juvenile body weight, a pair of White Leghorn chickens selected for high or low antibody response to sheep erythrocytes, and an F1 cross between each pair of lines, were used to produce DNA fingerprints (DFP). These DFP were prepared by mixing equal amounts of DNA from several individuals of a particular population, resulting in a DFP characteristic of the population. The populations provided individuals of known genetic relationships and inbreeding levels to evaluate the sensitivity of the DFP technique with DNA mixing. Levels of band sharing between breeds were lowest, those between selected lines within a breed were intermediate, and those between the selected lines and their F1 crosses were highest. These results show that DFP analysis is sensitive to several levels of genetic relationship.
选用一对针对幼年体重高或低进行选育的白普利茅斯洛克鸡品系、一对针对对绵羊红细胞抗体反应高或低进行选育的白来航鸡品系，以及每对品系之间的F1杂交后代，来制作DNA指纹图谱（DFP）。这些DFP是通过将特定群体中几个个体的等量DNA混合制备而成的，从而产生该群体特有的DFP。这些群体提供了具有已知遗传关系和近亲繁殖水平的个体，以评估DFP技术对DNA混合的敏感性。品种间的条带共享水平最低，同一品种内选育品系间的条带共享水平居中，而选育品系与其F1杂交后代间的条带共享水平最高。这些结果表明，DFP分析对几种遗传关系水平敏感。
