Larina O N
Institute of Biomedical Problems, Moscow, USSR.
Physiologist. 1991 Feb;34(1 Suppl):S94-5.
The two-dimensional plasma protein map of crewmembers of long-duration "Mir" expeditions obtained the day after the recovery shows a manifold increase in the content of several proteins normally seen in trace amounts. The emergence of several unusual protein spots occurs as well, some of them probably due to charge shifts provided by the events influencing posttranslational modification processes. By the 8 postflight day these phenomena were disappeared. In the "Cosmos-1887" biosatellite experiment, the plasma samples obtained two days after the landing as well as plasma of synchronous animals exhibited the higher fibrinogen levels when compared to those of vivarium animals. The protein consisting of a number of fractions with molecular weight of 50 to 60 kD and pI 5 to 6 had protein spots of similar size in flight and synchronous animals while in vivarium rats one of the spots was larger in size as opposed to the others. The plasma protein spectrum of flight and synchronous groups of animals in "Cosmos-1887" experiment where plasma samples were prepared in the period of time from 5 to 10 hours after spaceflight coincided with the pattern of vivarium animals. The data suggest that the protein changes described above develop during postflight period and accelerations, vibrations, readaptation to 1 G gravity, emotional stress could be the cause of these alterations.
在“和平号”长期考察队队员返回后的第二天获得的二维血浆蛋白质图谱显示，几种通常含量极少的蛋白质含量大幅增加。还出现了几个异常的蛋白质斑点，其中一些可能是由于影响翻译后修饰过程的事件导致的电荷转移。到飞行后第8天，这些现象消失了。在“宇宙-1887”生物卫星实验中，着陆两天后采集的血浆样本以及同步动物的血浆与饲养室动物的血浆相比，纤维蛋白原水平更高。由分子量为50至60 kD、等电点为5至6的多个组分组成的蛋白质在飞行动物和同步动物中具有大小相似的蛋白质斑点，而在饲养室大鼠中，其中一个斑点比其他斑点大。在“宇宙-1887”实验中，在航天飞行后5至10小时内制备血浆样本的飞行组和同步组动物的血浆蛋白质谱与饲养室动物的模式一致。数据表明，上述蛋白质变化发生在飞行后阶段，加速度、振动、重新适应1G重力、情绪压力可能是这些变化的原因。
