Li Qian, Guan Yue, Ma Hui-jie, Guo Zan, He Rui-rong, Zhang Yi
Department of Physiology, Hebei Medical University, Shijiazhuang 050017, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2010 Feb;26(1):117-20.
To observe the influences of Panax notoginsenosid(a compound of Chinese Traditional Medicine) on the spontaneous contraction of small intestine smooth muscle of rabbits in vitro and explore the mechanism.
The influences of Panax notoginsenosid on the spontaneous contraction of small intestine in intacted rabbits(male or female) after the isothermal perfuse of small intestine in vitro were observed. Bay K8644 and nitro-L-arginine methylester (L-NAME) were added to the normal Tyrode's solution respectively before Panax notoginsenosid. In the Ca2+ free Tyrode's solution, rynodine was added before Panax notoginsenosid. The mechanism of Panax notoginsenosid was studied.
Panax notoginsenosid reduced the amplitude of contraction of small intestine smooth muscle in rabbits in a does-depended manner. Bay K8644 and L-NAME could completely block the inhibition of Panax notoginsenosid on the contraction of small intestine smooth muscle. Panax notoginsenosid inhibited significantly the intracellular calcium-depended contraction induced by rynodine in the Ca2+ free Tyrode's solution.
Panax notoginsenosid inhibits significantly the contraction of small intestine smooth muscle of rabbits in vitro. The mechanism may be related to increase NO concentration in small intestine smooth muscle so that inhibit extracellular Ca2+ inflowing via cell membrane and intracellular Ca2+ releasing via sarcoplasmic reticulum.
观察三七总皂苷（一种中药成分）对家兔离体小肠平滑肌自发收缩的影响并探讨其机制。
观察三七总皂苷对体外恒温灌注的家兔（雌雄不限）小肠自发收缩的影响。在加入三七总皂苷前，分别向正常台氏液中加入Bay K8644和硝基-L-精氨酸甲酯（L-NAME）。在无钙台氏液中，在加入三七总皂苷前加入雷诺丁，研究三七总皂苷的作用机制。
三七总皂苷以剂量依赖方式降低家兔小肠平滑肌的收缩幅度。Bay K8644和L-NAME可完全阻断三七总皂苷对小肠平滑肌收缩的抑制作用。三七总皂苷在无钙台氏液中可显著抑制雷诺丁诱导的细胞内钙依赖性收缩。
三七总皂苷可显著抑制家兔离体小肠平滑肌的收缩。其机制可能与增加小肠平滑肌中一氧化氮浓度，从而抑制细胞外钙经细胞膜内流和细胞内钙从肌浆网释放有关。
