金银花与黄芩对家兔小肠平滑肌收缩及电活动的影响

[Effects of Honeysuckle flower and Scutellaria Baicalensis Georgi on constraction and electric activity of rabbit small intestine smooth muscle].

作者信息

Song Shi-jun, Li Fang-fang, Xu Ying, Zhang Li-hua, Yuan Fang, Zhang Yi

机构信息

Department of Physiology, the College of Basic Medical Sciences, Hebei Medical University, Shijiazhuang 050017, China.

出版信息

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2009 Aug;25(3):344-8.

PMID:21155231

Abstract

AIM

To investigate the effect of Honeysuckle flower (HF) and Scutellaria Baicalensis Georgi (SBG) on contraction and electric activity of small intestine smooth muscle in rabbit and the underlying mechanisms.

METHODS

Using organ bath technique to observe the effect of HF and SBG on contractive and electric activity of small intestine smooth muscle in rabbit.

RESULTS

HF and SBG significantly decreased the amplitude, frequency and area under the curve of contractive, as well as electric activity in a dose-depended manner. IC50 of the contractive amplitude was 6.30 g/L and 1.56 g/L by Logit Loglinear analysis. The inhibitive effect of HF and SBG on contractive activity could be partly decreased by beta-receptor blocker Propranolol, NO synthase inhibitor L-NAME, and K+ channel blocker Glibenclamide. Also HF and SBG inhibited acetylcholine-induced both intracellular and extracellular calcium-depended contraction significantly.

CONCLUSION

HF and SBG obviously inhibit the contractive and electric activity of small intestine smooth muscle of rabbit. The mechanisms are related to several pathways.

摘要

目的

研究金银花（HF）和黄芩（SBG）对家兔小肠平滑肌收缩及电活动的影响及其潜在机制。

方法

采用离体器官浴槽技术观察HF和SBG对家兔小肠平滑肌收缩及电活动的影响。

结果

HF和SBG能显著降低小肠平滑肌收缩的幅度、频率及收缩曲线下面积，同时也能显著降低电活动，且呈剂量依赖性。通过Logit Loglinear分析得出收缩幅度的半数抑制浓度（IC50）分别为6.30 g/L和1.56 g/L。HF和SBG对收缩活动的抑制作用可被β受体阻滞剂普萘洛尔、一氧化氮合酶抑制剂L-精氨酸甲酯（L-NAME）及钾通道阻滞剂格列本脲部分减弱。此外，HF和SBG能显著抑制乙酰胆碱诱导的细胞内及细胞外钙依赖性收缩。